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目的:探讨电刺激迷走神经对内毒素血症大鼠肠道炎症反应的影响。方法:雄性 Wistar 大鼠随机分为4组。实验组大鼠分离并切断双侧迷走神经,静注脂多糖(LPS)后持续刺激左侧迷走神经20min,间隔10min 后重复刺激20min,共刺激3次,对照组无电刺激。分别于静注 LPS 后1h、1.5h 和2h 处死动物,测定肠组织 TNF-α、髓过氧化物酶(MPO)含量,并观察病理形态学改变。结果:迷走神经刺激组肠组织 TNF-α、MPO 含量显著低于 LPS 组和迷走神经切断复合 LPS 组(P<0.05),组织炎性损害明显减轻。结论:迷走神经兴奋能显著减轻内毒素血症大鼠肠道局部炎症反应,其机制可能与对抗致炎因子有关。
Objective: To investigate the effects of electrical stimulation of vagus nerve on intestinal inflammatory response in endotoxemic rats. Methods: Male Wistar rats were randomly divided into 4 groups. Rats in experimental group were isolated and cut off bilateral vagus nerve, left vagus nerve was stimulated continuously with intravenous injection of lipopolysaccharide (LPS) for 20 minutes, and repeated stimulation for 10 minutes after 10 minutes interval was co-stimulated for 3 times without any electrical stimulation in the control group. Animals were sacrificed at 1h, 1.5h and 2h after intravenous injection of LPS, respectively. The content of TNF-α and MPO in intestinal tissue were measured, and the pathomorphological changes were observed. Results: The levels of TNF-α and MPO in intestinal mucosa of vagus nerve stimulation group were significantly lower than those of LPS group and vagotomy-combined LPS group (P <0.05), and inflammatory injury was significantly alleviated. Conclusion: The excitement of the vagus nerve can significantly reduce the intestinal inflammatory response in endotoxemic rats, which may be related to the anti-inflammatory cytokines.