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目的探讨丁酸钠预处理后内毒素性肝损伤小鼠肝脏的病理改变及其对炎症因子表达的影响。方法昆明小鼠72只随机分为6组,每组12只。联苯双酯组(联苯双酯溶液,剂量为200 mg·kg-1·d-1),丁酸钠低剂量组(丁酸钠溶液300 mg·kg-1·d-1)、中剂量组(丁酸钠溶液600 mg·kg-1·d-1)和高剂量组(丁酸钠溶液1 200 mg·kg-1·d-1),正常组(等容积生理盐水)和模型组(等容积生理盐水)均灌胃给药,1次/d,连续7 d。于给药第8天,除正常组外,各组通过脂多糖建立内毒素肝损伤模型,造模后6 h,处死小鼠取肝脏,HE染色观察肝脏病理学改变,实时聚合酶链反应(Real-time PCR)分析肝脏肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、白细胞介素(IL)-4和IL-10的mRNA水平。结果与正常组和联苯双酯组相比,模型组小鼠肝细胞出现肿胀、大量炎性细胞浸润、肝索结构紊乱等肝损伤病理学改变。与模型组相比,丁酸钠各剂量组小鼠肝细胞肿胀明显减轻,炎性细胞浸润减少,肝索结构较正常。与正常组小鼠相比,模型组的小鼠肝组织促炎因子INF-α、IFN-γ和抗炎因子IL-4、IL-6mRNA表达水平均增高(P均<0.01)。与模型组相比,丁醇钠低、中和高剂量组TNF-α和IFN-γmRNA的表达水平降低(P均<0.01),而抗炎因子IL-4和IL-10 mRNA的表达水平增高(P均<0.01),但丁酸钠各剂量组之间差异无统计学意义(P均>0.05)。结论丁酸钠可能通过下调促炎因子的表达和上调抗炎因子的表达保护内毒素性肝损伤。
Objective To investigate the pathological changes of liver in mice with endotoxic liver injury induced by sodium butyrate and its effect on the expression of inflammatory cytokines. Methods 72 Kunming mice were randomly divided into 6 groups of 12 rats. Bifendate group (bifendate solution, 200 mg · kg-1 · d-1), sodium butyrate low dose group (sodium butyrate solution 300 mg · kg-1 · d-1) (Sodium butyrate 600 mg · kg -1 · d -1) and high dose group (sodium butyrate 1200 mg · kg -1 · d -1), normal group (normal saline) and model Group (equal volume of saline) were intragastric administration, 1 / d, for 7 days. On the 8th day after administration, except for the normal group, lipopolysaccharide (LPS) -induced liver injury model was established in each group. After 6 h, the mice were sacrificed to take the liver, HE staining was used to observe the liver pathological changes, real-time polymerase chain reaction Real-time PCR was used to analyze the mRNA levels of liver tumor necrosis factor (TNF) -α, interferon (IFN) -γ, interleukin (IL) -4 and IL-10. Results Compared with the normal group and the bifendate group, the hepatocytes in the model group showed pathological changes such as swelling, a large number of inflammatory cell infiltration and liver structural disorder. Compared with the model group, each dose of sodium butyrate significantly reduced liver cell swelling, inflammatory cell infiltration decreased hepatic cord structure than normal. Compared with the normal mice, the levels of proinflammatory cytokines INF-α, IFN-γ and anti-inflammatory cytokines IL-4 and IL-6 mRNA in the model group were significantly increased (all P <0.01). Compared with model group, the expression levels of TNF-α and IFN-γmRNA in low, middle and high dose of sodium butoxide group decreased (P <0.01), but the expression of IL-4 and IL-10 mRNA increased (All P <0.01), but there was no significant difference between each dose of sodium butyrate (P> 0.05). Conclusion Sodium butyrate may protect endotoxin-induced liver injury by downregulating the expression of proinflammatory cytokines and upregulating the expression of anti-inflammatory cytokines.