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目的探讨转化生长因子β1(TGF-β1)、Ⅲ型前胶原端肽(PⅢNP)在接尘工人中表达水平的变化及其意义,探讨其作为尘肺早期诊断指标的可能性。方法采用双抗体夹心法,测定69例煤矿接尘工人和45例健康对照者血清中TGF-β1、PⅢNP的表达水平。结果接尘工人血清中TGF-β1、PⅢNP含量分别为(56.85±15.03)、(20.17±23.54)ng/ml,对照组则分别为(18.67±4.48)、(11.72±3.52)ng/ml,接尘组与对照组血清中TGF-β1、PⅢNP含量差异均有统计学意义(P<0.05),尘肺病患者血清中TGF-β1、PⅢNP含量随着接尘工龄的增加而降低,差异有统计学意义(P<0.05);两变量相关分析显示TGF-β1,与PⅢNP呈正相关关系(P<0.05)。结论血清中TGF-β1、PⅢNP表达与接触煤尘有关,细胞因子之间血清水平亦呈正相关,因此,2种细胞因子的异常表达可以作为尘肺病早期诊断的指标之一。
Objective To investigate the expression and significance of TGF-β1 and PⅢNP in workers exposed to dust, and to explore the possibility of using TGF-β1 as an early diagnostic indicator of pneumoconiosis. Methods Double antibody sandwich method was used to detect the expression of TGF-β1 and PⅢNP in serum of 69 mine-exposed workers and 45 healthy controls. Results The serum levels of TGF-β1 and PⅢNP in workers exposed to dust were (56.85 ± 15.03) and (20.17 ± 23.54) ng / ml respectively, while those in the control group were (18.67 ± 4.48) and (11.72 ± 3.52) ng / The levels of TGF-β1 and PⅢNP in the serum of the dust group and the control group were significantly different (P <0.05). The contents of TGF-β1 and PⅢNP in the serum of the pneumoconiosis patients decreased with the increase of the service life of dust, the differences were statistically significant (P <0.05). Correlation analysis between two variables showed that there was a positive correlation between TGF-β1 and PⅢNP (P <0.05). Conclusions The expression of TGF-β1 and PⅢNP in serum is related to the contact with coal dust and the serum level of cytokines is also positively correlated. Therefore, abnormal expression of the two cytokines can be used as one of the indicators of early diagnosis of pneumoconiosis.