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为探明本地区耐甲氧西林金黄色葡萄球菌(Staphylococcusaureus)的耐药性、流行病学分布状况及携带的葡萄球菌染色体mec盒(SCCmec)型别,用K-B琼脂扩散法、E-test和多位点PCR,对临床分离的金黄色葡萄球菌菌株进行了SCCmec分型及耐药性测定。结果发现了两种新的SCCmec型别,新1型含Ⅱ型的mecA上游特异性位点B和位于mecA内的M位点以及Ⅲ型的下游位点F,缺乏Ⅱ型上游位点C和下游位点D、G;新2型含Ⅰ、Ⅱ型的上游特异性位点A、B和两个Ⅲ型的下游位点F、H,同样缺乏位点C、D、G,可能分别为原有Ⅱ型和Ⅰ、Ⅱ型与Ⅲ型的基因重组株;且携带有新SCCmec型别的MRSA菌株,其流行病学分布特点及抗药性也与国外已报导的菌株不同,多分自门诊病人,且耐药性高,抗药谱广,值得引起高度重视和关注。
In order to explore the resistance, epidemiological distribution and staphylococcus mec box (SCCmec) type of methicillin-resistant Staphylococcus aureus in this area, KB agar diffusion method, E-test and Multi-site PCR, the clinical isolates of Staphylococcus aureus SCCmec typing and drug resistance determination. As a result, two new types of SCCmec were found, including type I mecA upstream site B, type M mecA site, type III downstream site F, type II upstream site C and Downstream sites D, G; the new type 2 containing type Ⅰ, Ⅱ upstream specific sites A, B and two type Ⅲ downstream sites F, H, the same lack of sites C, D, G may be The original type Ⅱ and Ⅰ, Ⅱ and Ⅲ type of gene recombinant strains; and strains carrying the new SCCmec type MRSA strains, the epidemiological characteristics and drug resistance and foreign reports have also been different strains, more outpatients , And high resistance, broad spectrum of resistance, it deserves a high degree of attention and attention.