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目的:对2008年崇明地区发生的霍乱疫情中分离的和平时监测中分离的霍乱菌株进行遗传相关性分析,追溯传染源,为霍乱疫情的预测和制定防治措施提供依据。方法:PCR检测霍乱弧菌毒力基因;脉冲场凝胶电泳(PFEG)对实验菌株进行分子分型,并分析结果。结果:所试8株菌株均为毒力阳性,均为产毒株。8株菌的PFGE为5个型。结论:8株O139群霍乱弧菌均为产毒株,具有致病性。崇明地区首次在被污染的苍蝇中分离出霍乱弧菌,经流调排除其为传染源,但要加强该环节的监测力度。2008年度崇明发生的4起霍乱疫情从分子生物学角度分析为4起独立散发的疫情,无关联性。
OBJECTIVE: To carry out genetic correlation analysis of the cholera isolates isolated during peacetime surveillance isolated from cholera epidemics in 2008 in Chongming area, trace the source of infection, and provide the basis for the prediction of cholera epidemic and the development of prevention and control measures. Methods: Virulence genes of Vibrio cholerae were detected by PCR. Molecular typing of the experimental strains was carried out by pulsed-field gel electrophoresis (PFEG). The results were analyzed. Results: All the 8 tested strains were virulent, all of them were producing strains. PFGE of 8 strains were 5 types. Conclusion: All 8 strains of Vibrio cholerae O139 are pathogenic. For the first time in Chongming, Vibrio cholera was isolated from contaminated flies, which was ruled out as a source of infection by flow tempering. However, monitoring should be stepped up. Four cholera outbreaks in Chongming in 2008 were analyzed from the perspective of molecular biology as four separate outbreaks of epidemics with no correlation.