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目的探讨人参总甙、人参皂甙单体对脐血CD 34+细胞体外扩增的影响。方法用StemSpanTMSFEM无血清液体培养基加入不同浓度的人参总皂甙 (GS)、人参皂甙单体 (Rg1、Rb1) ,进行液体扩增 ,计数总细胞数并检测CD 34+细胞。收获扩增后的细胞用MethoCultTMH 4 4 35半固体培养基培养 ,计数CFU E、BFU E、CFU GM集落。结果GS、Rg1、Rb1具有刺激CD 34+细胞液体培养的作用。GS的最佳浓度是 5 0mg/L ,Rg1最佳浓度是 5mg/L ,Rb1最佳浓度是 5mg/L。细胞总数分别扩增 2 0 .4、2 4 .5、2 4 .1倍 ,CD 34+细胞数分别扩增 4 .2 2、5 .19、4 .12倍。在液体培养后仍可刺激扩增后脐血细胞集落形成 ,CFU E集落形成能力分别比对照组高 90 .1%、70 .8%、72 .8% ;BFU E集落形成能力分别比对照组高 5 3.4 %、6 8.0 %、6 7.0 % ;CFU GM集落形成能力分别比对照组高 6 7.0 %、2 9.8%、39.4 % ;结论人参总甙、人参皂甙单体能促进造血干 /祖细胞增殖 ,并且能诱导定向分化 ,具有类似生长因子和协同生长因子作用。
Objective To investigate the effects of ginsenosides and ginsenosides on the in vitro expansion of cord blood CD34+ cells. Methods StemSpanTM SFEM serum-free liquid medium was added to different concentrations of ginsenosides (GS) and ginsenosides (Rg1, Rb1) for liquid amplification. The total cell number was counted and CD34+ cells were detected. Harvested and expanded cells were cultured in MethoCultTM H 4 4 35 semi-solid medium, and colonies of CFU E, BFU E, and CFU GM were counted. As a result, GS, Rg1, and Rb1 had the effect of stimulating the liquid culture of CD34+ cells. The optimal concentration of GS is 50 mg/L, the optimal concentration of Rg1 is 5 mg/L, and the optimal concentration of Rb1 is 5 mg/L. The total number of cells was amplified by 20.4, 24.5, and 24.1 folds respectively, and the number of CD34+ cells was amplified by 4.22, 5.19, and 4.12 folds, respectively. After colony culture, umbilical cord blood cell colony formation could still be stimulated. The colony forming ability of CFU E was 90.1%, 70.8%, and 72.8% higher than that of the control group. The colony forming ability of BFU E was higher than that of the control group. 5 3.4 %, 6 8.0%, 67.0 %; CFU GM colony forming ability was higher than the control group by 67.0 %, 29.8% and 39.4% respectively; Conclusion Ginsenoside and ginseng saponin monomer can promote the proliferation of hematopoietic stem/progenitor cells And can induce directed differentiation with similar growth factors and co-growth factors.