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目的 观察非甾体类抗炎药、环氧合酶(COX)-2抑制剂吡罗昔康(Piroxicam)对结肠癌细胞的影响,并结合COX-2蛋白表达和细胞凋亡情况探讨结肠癌的预防。方法 细胞株选用SW1116结肠腺癌细胞;细胞增殖实验时,用MTT法测定细胞增殖活性;用免疫组化及Western Blot方法检测细胞内COX-2蛋白表达;用DNA云梯电泳法检测细胞凋亡。结果 吡罗昔康能够抑制结肠腺癌细胞的增殖,其效应与浓度呈正相关;浓度≥1.0 mmol/L时呈现细胞毒作用。吡罗昔康作用12 h即可显著抑制COX-2蛋白的表达;作用24 h后,蛋白水平恢复程度与浓度成负相关。吡罗昔康浓度≥0.1 mmol/L时可以诱导SW 1116细胞的凋亡。结论 吡罗昔康抑制结肠腺癌细胞的增殖与抑制COX-2过度表达和促进细胞凋亡有关,由于吡罗昔康的效应呈现浓度和时间依赖性,在进行预防或治疗结肠癌的临床研究时,要考虑其有效剂量和用药间隔。
Objective To investigate the effect of non-steroidal anti-inflammatory drugs (NSAIDs) and cyclooxygenase (COX) -2 inhibitor Piroxicam on human colon cancer cells. Combine the expression of COX-2 protein and apoptosis, . Methods SW1116 colon adenocarcinoma cells were selected as the cell lines. Cell proliferation was measured by MTT assay. The expression of COX-2 protein was detected by immunohistochemistry and Western Blot. Cell apoptosis was detected by DNA ladder electrophoresis. Results Piroxicam can inhibit the proliferation of colon adenocarcinoma cells, and its effect is positively correlated with the concentration. When the concentration is greater than or equal to 1.0 mmol / L, it shows cytotoxicity. The effect of piroxicam for 12 h significantly inhibited the expression of COX-2 protein; after 24 h, the level of protein recovery was negatively correlated with the concentration. Piroxicam at a concentration of 0.1 mmol / L induces apoptosis in SW1116 cells. Conclusion Piroxicam inhibits the proliferation of colon adenocarcinoma cells and inhibits the overexpression of COX-2 and promotes cell apoptosis. Due to the concentration-dependent and time-dependent effects of piroxicam, it is necessary to consider when conducting clinical studies on the prevention or treatment of colon cancer The effective dose and medication interval.