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目的观察和分析BALB/c小鼠自身免疫性睾丸炎后病理改变及肥大细胞(MC)的数量、分布等变化。方法 BALB/c雄性小鼠72只,随机分为6组,每组12只,其中5组为模型组,行单侧睾丸动脉结扎,在结扎后2h开通血管,分别在开通后1 2h、1周、2周、3周、4周处死,取睾丸和附睾;另一组为对照组,不做任何处理,直接取睾丸和附睾进行对比。结果 TB染色显示对照组侧睾丸MC基本上只见于白膜下面,睾丸间质中几乎不存在或仅偶见MC。模型组侧小鼠睾丸白膜、睾丸间质、睾丸纵隔以及附睾中均可见MC。其中附睾中MC的数量最多,依次是纵隔、白膜、睾丸间质。模型组MC的数量和分布与对照组比较差异具有显著性。HE染色显示:随着缺血后再灌注的时间延长,组织病理变化逐渐减轻。结论自身免疫性睾丸炎模型组MC数量高于对照组,睾丸组织病理变化随时间延长逐渐减轻,具有相对的可恢复性。
Objective To observe and analyze the pathological changes and the number and distribution of mast cells (MC) in BALB / c mice after autoimmune orchitis. Methods 72 male BALB / c male mice were randomly divided into 6 groups (12 rats in each group), including 5 rats in the model group. Unilateral testicular artery ligation was performed. Blood vessels were opened 2 h after ligation, Week, 2 weeks, 3 weeks, 4 weeks sacrifice, testis and epididymis; the other group as the control group, without any treatment, directly take the testis and epididymis for comparison. Results TB staining showed that MC in the testis side of the control group was almost only found under the albuginea, with little or only occasional MC in the interstitium of the testis. Model group side of the mouse testicular white matter, testicular stroma, testicular mediastinal and epididymides are visible MC. One of the largest number of epididymal MC, followed by mediastinal, white film, testicular interstitial. The number and distribution of MC in the model group were significantly different from those in the control group. HE staining showed that with the reperfusion time after ischemia, histopathological changes gradually reduced. Conclusions The number of MC in autoimmune orchitis model group is higher than that in control group. The histopathological changes of testicular tissue gradually lighten with time and have relatively recoverability.