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目的探讨神经节苷脂对脑缺血再灌注损伤的保护作用。方法采用Ｐｕｌｓｉｎｅｌｌｉ的四血管闭塞法 制作大鼠急性脑缺血／再灌注模型，利用高压液相色谱法观察脑缺血／再灌注期间谷氨酸和γ－氨基丁酸含量的变化， 通过图象分析仪测定神经元的面数密度及神经节苷脂对以上指标的影响。结果缺血后谷氨酸和γ－氨基丁酸含 量明显升高，再灌注后降低，神经节苷脂可降低谷氨酸含量，与盐水对照组相比差异显著（Ｐ＜０．０１），同时增加神经 元面数密度。结论神经节苷脂通过抑制谷氨酸释放，可减轻脑缺血再灌注期间脑组织损伤。
Objective To investigate the protective effect of gangliosides on cerebral ischemia-reperfusion injury. Methods Acute cerebral ischemia / reperfusion model was established by four-vessel occlusion method in Pulsinelli. The contents of glutamic acid and γ-aminobutyric acid during cerebral ischemia / reperfusion were observed by high pressure liquid chromatography. Analyzer to determine the number density of neurons and ganglioside on the above indicators. Results Glutamate and γ-aminobutyric acid were significantly increased after ischemia and decreased after reperfusion. Ganglioside decreased glutamate content compared with saline control group (P <0.01) At the same time increase the number density of neurons. Conclusion Gangliosides can relieve brain injury during cerebral ischemia / reperfusion by inhibiting glutamate release.