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【目的】了解小麦骨干亲本小偃6号及其衍生品种(系)间的遗传差异,以及小偃6号对各代衍生品种的遗传贡献。【方法】利用63对分布于小麦21条染色体上的SSR引物对小偃6号及其子1代到子6代的80个衍生品种(系)进行UPGMA聚类分析,以各衍生品种与小偃6号共有位点数占总位点数的百分比表示小偃6号对各世代的遗传贡献。【结果】63对SSR引物在81份材料中共检测到175个等位变异,等位变异数目为1~6,平均2.78个;SSR引物多态性信息含量的变化幅度为0.067 4~0.836 4,平均值为0.480 4;品种间遗传相似系数变化幅度为0.521~0.931,平均值为0.664,在遗传相似系数为0.658处将81份材料分为7类,聚类结果与系谱较吻合;小偃6号对其衍生子1代、子2代、子3代、子4代的平均遗传贡献率为50.32%,47.54%,46.35%,44.83%;在各世代中A、B和D基因组间遗传贡献率整体表现为D>A>B。【结论】小偃6号与其衍生品种(系)存在一定的遗传差异;小偃6号在前4个世代对各世代的遗传贡献率随世代的增加逐渐下降,对衍生品种(系)的D基因组遗传贡献较大。
【Objective】 The objective of this study was to understand the genetic differences among the wheat parents Xiaoyan 6 and its derivative lines (lines) and the genetic contribution of Xiaoyan 6 to the derivative lines of each generation. 【Method】 UPGMA was used to analyze 80 derivative cultivars (lines) of Xiaoyan No. 6 and its first generation to the sixth generation using 63 pairs of SSR primers distributed on 21 chromosomes of wheat. By using UPGMA cluster analysis, Yan 6 number of shared sites in the total number of sites that Xiaoyan 6 genetic contribution to each generation. 【Result】 A total of 175 SSR primers were detected in 81 accessions with the number of alleles ranging from 1 to 6, with an average of 2.78. SSR primer polymorphism information content ranged from 0.067 4 to 0.836 4, With an average of 0.480 4. The genetic similarity coefficients of cultivars ranged from 0.521 to 0.931 with an average of 0.664. The genetic similarity coefficient was 0.658, and 81 cultivars were divided into 7 groups. The clustering results were in good agreement with pedigree. The average genetic contribution rates of the first generation, the second generation, the third generation and the fourth generation of their derivatives were 50.32%, 47.54%, 46.35% and 44.83% respectively. The genetic contribution between A, B and D genomes in each generation The overall performance rate of D> A> B. 【Conclusion】 There was a certain genetic difference between Xiaoyan 6 and its derivative varieties (lines). The genetic contribution of Xiaoyan 6 to each generation in the first 4 generations decreased gradually with increasing generations, Genomic genetic contribution.