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珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatu♂)是鞍带石斑鱼(♂E.lanceolatu)与棕点石斑鱼(♀E.fuscoguttatus)杂交出的新品种。本研究用共轭亚油酸(CLA)添加量为0(CLA0)、0.8%(CLA0.8)、1.6%(CLA1.6)、2.4%(CLA2.4)、3.2%(CLA3.2)的5种等氮等能饲料,投喂初始体重为(63.57±0.56)g珍珠龙胆石斑鱼幼鱼8周,研究不同共轭亚油酸水平对其生长、体组成及肝代谢相关酶活的影响。结果表明:(1)添加共轭亚油酸对实验鱼增重率、成活率、特定生长率、肥满度、肝体比和脏体比无显著影响;蛋白质效率随着饲料中共轭亚油酸水平的升高而升高,其他各组显著高于CLA0组(P<0.05);摄食率随着饲料中共轭亚油酸水平的升高而降低,CLA0组显著高于其他各组(P<0.05);饲料系数呈降低趋势,在CLA2.4组达到最低,显著低于CLA0组(P<0.05)。(2)肌肉脂肪含量随饲料CLA水平的升高而升高,除CLA0.8组外,其他各组显著高于CLA0组(P<0.05),肝脂肪含量随着饲料CLA含量的变化未见显著性差异。CLA1.6组肌肉中饱和脂肪酸和C_(18:0)含量显著高于CLA0组(P<0.05),CLA3.2组单不饱和脂肪酸和C_(18:1n-9)显著低于CLA0组(P<0.05)。CLA2.4组和CLA3.2组肝中C_(16:0)和C_(18:0)含量显著高于CLA0组(P<0.05),而C_(16:1n-7)、C_(18:1n-9)和C_(18:1n-7)含量显著低于CLA0组(P<0.05)。CLA2.4组和CLA3.2组肌肉和肝中C_(18:3n-3)和C_(18:2n-6)在显著低于CLA0组(P<0.05)。肌肉和肝中C_(18:2c9,t11)和C_(18:2t10,c12)含量同共轭酸添加水平呈现高度正相关,且各添加组均显著高于CLA0组(P<0.05)。(3)饲料中共轭亚油酸水平影响葡萄糖-6-磷酸脱氢酶(G6PD)的活性,各实验组均显著高于CLA0组(P<0.05);饲料中共轭亚油酸水平影响酰基辅酶A氧化酶(ACO)的活性,其中CLA3.2活性最高,均高于其他组(P<0.05);除CLA0.8组的总酯酶和脂肪酸合成酶活力低于CLA0组外,其他各组均高于CLA0组(P<0.05)。实验结果表明,日粮中适量添加CLA可显著提高珍珠龙胆石斑鱼幼鱼对饲料的利用,改善肌肉和肝中脂肪酸组成,同时增加肝脂肪代谢相关酶的活性。
Epinephelus fuscoguttatus♀ × E.lanceolatu♂ is a new hybrid between ♂E.lanceolatu and E.fuscoguttatus. In this study, CLA was supplemented with CLA 0. 0.8% CLA 1.6 1.6% 2.4% CLA 3.2% Of five kinds of nitrogen and other feed can feed the initial weight (63.57 ± 0.56) g juvenile Gentiana grouse juvenile eight weeks to study the different levels of conjugated linoleic acid on its growth, body composition and liver metabolism related enzymes Impact. The results showed that: (1) There was no significant effect of adding conjugated linoleic acid on the weight gain rate, survival rate, specific growth rate, fatness, liver body ratio and dirty body ratio of experimental fish; (P <0.05). The feed intake rate decreased with the increase of conjugated linoleic acid in feed, the level in CLA0 group was significantly higher than that in other groups (P <0.05) <0.05). The feed coefficient showed a decreasing trend, which reached the lowest in CLA2.4 group and significantly lower than CLA0 group (P <0.05). (2) Muscle fat content increased with the increase of dietary CLA levels, except CLA0.8 group, the other groups were significantly higher than the CLA0 group (P <0.05), liver fat content with the changes of dietary CLA Significant difference. The content of saturated fatty acid and C_ (18_0) in CLA1.6 group was significantly higher than that in CLA0 group (P <0.05). The content of monounsaturated fatty acids and C_ (18_1n_9) in CLA3.2 group was significantly lower than that in CLA0 group P <0.05). The C_ (16: 0) and C_ (18_0) contents in the liver of CLA2.4 group and CLA3.2 group were significantly higher than those in CLA0 group (P <0.05), while those of C_ (16: 1n_7) 1n-9) and C_ (18: 1n-7) were significantly lower than CLA0 group (P <0.05). C_ (18: 3n-3) and C_ (18: 2n-6) in CLA2.4 group and CLA3.2 group were significantly lower than those in CLA0 group (P <0.05). The contents of C_ (18: 2c9, t11) and C_ (18: 2t10, c12) in muscles and liver were highly positively correlated with the addition of conjugate acid, and the contents of C_ (18: 2c9, (3) The level of conjugated linoleic acid in diet affected the activity of glucose-6-phosphate dehydrogenase (G6PD), which was significantly higher in each experimental group than that in CLA0 group (P <0.05). Conjugated linoleic acid (P <0.05). The activities of total lipase and fatty acid synthase in CLA0.8 group were lower than those in CLA0 group Higher than CLA0 group (P <0.05). The results showed that proper dietary supplementation of CLA significantly increased feed utilization, improved fatty acid composition in muscle and liver, and increased hepatic lipase-related enzyme activity.