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目的:考察枳实-芍药药对中主成分柚皮苷、橙皮苷、新橙皮苷分别和芍药苷配伍后相应成分的肠道吸收特点。方法:采用大鼠在体肠灌流模型,超高效液相色谱(UPLC)测定灌流液中柚皮苷、橙皮苷、新橙皮苷和芍药苷的含量,计算有效渗透系数Peff及10cm肠段吸收百分比(10%ABS),比较主成分配伍前后化合物在肠道吸收的差异。结果:柚皮苷、橙皮苷、新橙皮苷分别与芍药苷不同比例配伍后各成分在大鼠十二指肠、空肠、回肠和结肠4个肠段中的Peff值和10cm%ABS值与单个成分相比无显著性变化(P>0.05),且配伍比例的变化并不显著影响主成分的吸收(P>0.05)。结论:与单体成分的肠吸收相比,枳实-芍药药对中主成分配伍并不能显著性影响主成分的肠吸收。
OBJECTIVE: To study the intestinal absorption of the corresponding components after the combination of Zhizhi-Shaoyao, the main ingredient of naringin, hesperidin, neohesperidin and paeoniflorin. Methods: The contents of naringin, hesperidin, neohesperidin and paeoniflorin in perfusate were determined by rat intestinal perfusion model and ultra-high performance liquid chromatography (UPLC), and the effective permeability coefficient Peff and 10cm intestinal segment Percent Absorption (10% ABS) to compare differences in intestinal absorption of compounds before and after compatibility of the main components. Results: The Peff value and 10cm% ABS value of naringin, hesperidin and neohesperidin with different proportions of paeoniflorin, respectively, in the intestine of duodenum, jejunum, ileum and colon of rats Compared with the single component, there was no significant change (P> 0.05), and the change of the compatibility ratio did not significantly affect the absorption of the main component (P> 0.05). CONCLUSION: Compared with the intestinal absorption of monomer components, the combination of Zhizhi - Shaoyao on the main components does not significantly affect the intestinal absorption of the main components.