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利用基因诱捕载体整合到人类肝癌细胞系SMMC7721细胞的染色体基因中,得到永久性高表达诱捕载体报告基因X-gal的阳性克隆,再用Cre-LoxP置换系统,将构建好的HBV-P全长片段与诱捕载体的报告基因部分交换,HBV-P全长片段完整的整合在SMMC7721细胞的染色体基因中,得到了稳定表达HBV-P蛋白的细胞系。该细胞系为制备、纯化P抗原和研究HBV-P的基因调控提供了实验材料。
The gene cloning vector was integrated into the chromosomal gene of human hepatocellular carcinoma cell line SMMC7721 to obtain the positive cloning vector X-gal, which is a cloned vector. After that, the full-length HBV-P was constructed using the Cre-LoxP replacement system The fragment was exchanged with the reporter vector of the trapping vector, and the full-length fragment of HBV-P was integrated into the chromosomal gene of SMMC7721 cells to obtain a cell line stably expressing HBV-P protein. The cell line for the preparation, purification of P antigen and study of HBV-P gene regulation provides experimental materials.