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目的:分别观察不同含油量木鳖子霜急性毒性与药效学作用,探讨毒性与药效学的关系,为制霜工艺提供毒理和药效学依据。方法:按照急性毒性试验LD50测定方法,灌胃给予10%、20%、30%含油量木鳖子霜和木鳖子原药材的水混悬液,观察其急性毒性,Bliss法计算各自的LD50和95%可信度区间。采用小鼠耳肿胀法和小鼠热板法观察不同含油量木鳖子霜的抗炎、镇痛作用,观察小鼠体重、脏器指数、耳肿胀率、痛阈值等指标。结果:10%、20%、30%含油量木鳖子霜和原药材的LD50分别为3.923、5.6393、5.9594、6.1610g/kg。不同含油量木鳖子霜对小鼠体重、脏器指数、耳肿胀率、痛阈值等指标有不同程度影响,小鼠一般状况较空白对照组差,体重普遍低于空白对照组;对小鼠主要免疫器官具有不同程度的抑制作用;具有明确的抗炎和镇痛作用,但对镇痛试验的舔足次数无显著性影响。上述所有指标均未呈现明显的线性量效关系,随含油量的增加,均呈现出先增强后减弱的变化趋势,其中10%～20%含油量时其抗炎、镇痛作用最好,且对体重和免疫器官的影响最小。结论:木鳖子毒性随着含油量的增大呈现降低趋势,木鳖子在20%含油量时抗炎、镇痛等药效学作用最为明显,对免疫器官的抑制作用最小,对小鼠一般状况和体重影响最小。
OBJECTIVE: To observe the acute toxicity and pharmacodynamic effects of Momordicae selengensis oil with different oil content, and to explore the relationship between toxicity and pharmacodynamics, and to provide toxicological and pharmacodynamic evidences for the preparation of cream. Methods: According to acute toxicity test LD50, 10%, 20%, 30% oil-in-water aqueous suspension of Momordicae selengensis and Momordicae selenges was orally administered to observe its acute toxicity. The LD50 And 95% confidence interval. Anti-inflammatory and analgesic effects of Momordicaea Schisandrae with different oil content were observed by mouse ear edema method and mouse hot-plate method. Body weight, organ index, ear swelling rate and pain threshold were observed. Results: LD50 of 10%, 20%, 30% oil-containing Momordicae semen and original medicinal materials were 3.923,5.6393,5.9594,6.1610g / kg, respectively. Mucuna cream with different oil content had different effects on mice body weight, organ index, ear swelling rate and pain threshold. The general condition of mice was worse than blank control group and the body weight was generally lower than that of blank control group. The main immune organs have different degrees of inhibition; has a clear anti-inflammatory and analgesic effects, but analgesic test licking the number of no significant effect. All of the above indexes did not show a significant linear relationship between dose-effect, with the increase of oil content, showing a trend of first increasing and then weakening, of which 10% to 20% oil content of the best anti-inflammatory and analgesic effect, and Body weight and immune organs have the least impact. Conclusion: The toxicity of Momordicae sinensis decreased with the increase of oil content. The pharmacodynamic action of Momordica spp. At 20% oil content was the most obvious, with the least inhibitory effect on immune organs. In mice, The general condition and weight have the least impact.