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目的 探讨乌司他丁对活体肺缺血再灌注损伤的保护作用。方法 将 2 0只新西兰大白兔随机分成缺血再灌注损伤组 (A组 )和乌司他丁组 (B组 ) ,B组阻断前给乌司他丁 (1 0 0 0 0U/kg体重 )。两组阻断 2h和再灌注 1h后采血检测血气、白细胞介素 6(IL 6)和肿瘤坏死因子 α(TNF α)。摘取左肺测定湿干重比 (W/B)和病理学检查。结果 阻断 2h后 ,两组血氧分压 (PO2 )接近 ;恢复灌注 1h后 ,B组PO2 高于A组 (77.61± 5 .0 4 )mmHg(1mmHg =0 .1 33kPa)和 (1 0 0 .85± 6 .73)mmHg ;TNF α值 (A值 ) ,A组各时段均显著高于B组 (2 54 .0 2± 1 4 .31和 50 4 .0 2± 33 .52比 1 4 8.63± 2 1 .0 6)和 1 60 .54± 1 6 .93) ;A组肺湿干重比高于B组 ;肺外观苍白肿胀 ,病理检查见组织结构损伤。结论 乌司他丁能保护在体肺缺血再灌注中肺组织结构的损伤
Objective To investigate the protective effect of ulinastatin on lung ischemia-reperfusion injury in vivo. Methods Twenty New Zealand white rabbits were randomly divided into ischemia reperfusion injury group (A group) and ulinastatin group (B group), and group B was treated with ulinastatin (10 000 U / kg body weight ). Blood gas, interleukin 6 (IL 6) and tumor necrosis factor α (TNF α) were detected in both groups after blocking for 2 hours and reperfusion for 1 hour. Left lung was taken for determination of wet / dry weight ratio (W / B) and pathological examination. Results After 2 hours of blocking, the partial pressure of oxygen (PO2) was similar in both groups. After 1h of reperfusion, the PO2 in group B was significantly higher than that in group A (77.61 ± 5.04mmHg (1mmHg = 0.133kPa) and 0 .85 ± 6 .73) mmHg; TNFα value (A value) in group A was significantly higher than that in group B (542 ± 14.31 and 50 4 .0 2 ± 33.52 1 4 8.63 ± 2 1 .0 6) and 1 60 .54 ± 1 6 .93). The wet and dry weight ratio of lungs in group A was higher than that of group B. The appearance of lung was pale and swollen. The pathological examination revealed the tissue damage. Conclusion Ulinastatin can protect lung tissue from structural damage during lung ischemia-reperfusion