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[目的]观察氯化镨(PrCl3)对小鼠骨髓细胞微核率和核异常率的影响,以及亚硒酸钠(Na2SeO3)的干预作用。[方法]试验动物为5~6周龄昆明种小鼠;试验设置12个组,即5个氯化镨剂量组[7.5、15、30、60、120mg(/kg bw)]、5个亚硒酸钠干预组[在5个PrCl3剂量的基础上分别加入0.05mg(/kg bw)的亚硒酸钠]、2个对照组(阴性组为0.85%生理盐水,阳性组为叠氮化钠[(NaN3),20mg(/kg bw)对照组]。各组小鼠采用腹腔注射染毒或给药,运用微核测试技术和细胞核检测按照常规标准观察小鼠骨髓细胞的微核率(FMN)和核异常率(FAN),比较亚硒酸钠干预组对小鼠骨髓细胞FMN和FAN的影响。[结果]在7.5~120mg(/kg bw)的氯化镨剂量内,可致小鼠骨髓细胞FMN、FAN显著增高,并呈现剂量-效应关系(rFMN=0.885,rFAN=0.914);将亚硒酸钠干预组与氯化镨剂量组两两对应相比较,亚硒酸钠干预组的小鼠骨髓细胞FMN及FAN均有下降,经t检验显示,除7.5mg(/kg bw)组差异无统计学意义以外,其余4组差异均有统计学意义(P<0.05或0.01)。[结论]氯化镨提高了小鼠骨髓细胞核FMN及FAN,具有遗传损伤作用;加入亚硒酸钠能够降低小鼠细胞核的FMN及FAN,具有明显的干预作用。
[Objective] To observe the effects of PrCl3 on the micronucleus rate and nuclear abnormality of mouse bone marrow cells and the intervention of sodium selenite (Na2SeO3). [Method] The experimental animals were Kunming mice of 5 to 6 weeks old. Twelve groups were set up, namely, 5 doses of 7.5, 15, 30, 60 and 120 mg (/ kg bw) Sodium selenate intervention group [Add 0.05mg (/ kg bw) sodium selenite on the basis of 5 PrCl3 doses respectively), two control groups (negative group: 0.85% saline, positive group: sodium azide [(NaN3), 20mg (/ kg bw) control group] .Mice in each group were injected intraperitoneally with drugs or administered by micronucleus test and micronucleus test, and the micronucleus rate of mouse bone marrow cells (FMN ) And nuclear anomalous rate (FAN). The effects of sodium selenite intervention group on the FMN and FAN of mouse bone marrow cells were compared. [Results] Within the dose of 7.5 ~ 120mg (/ kg bw) The FMN and FAN of bone marrow cells were significantly increased, and showed a dose-effect relationship (rFMN = 0.885, rFAN = 0.914); sodium selenite intervention group and praseodymium chloride dose group two or two correspondence compared to sodium selenite intervention group The FMN and FAN of mice bone marrow cells decreased, the t test showed that except for the group of 7.5mg (/ kg bw), the differences were not statistically significant (P <0.05 or 0.01) Conclusion] Praseodymium chloride increased Murine myeloma cell nucleus and FMN FAN, genetic injury; sodium selenite was added FMN nuclei can be reduced in mice and FAN, obvious intervention.