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目的:探讨核糖体S6激酶4(RSK4)抑癌基因在人乳腺癌细胞株MDA-MB-231、T47D、Bcap37和MCF-7中的表达,并筛选出高和低表达RSK4基因的乳腺癌细胞。方法:逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法从mRNA和蛋白水平检测RSK4在MDA-MB-231、T47D、Bcap-37和MCF-7 4种乳腺癌细胞株中的表达。结果:人乳腺癌细胞株MDA-MB-231、T47D、Bcap37和MCF-7中均有RSK4基因表达,其中,MCF-7细胞RSK4表达水平最高,为表达量最低的MDA-MB-231细胞的2.9倍,MCF-7细胞RSK4蛋白表达水平也最高,与其他3种细胞株相比,差异有统计学意义,P<0.001。RT-PCR法和蛋白质印迹法检测结果一致,具有高度相关性,r=0.766,P<0.001。结论:人乳腺癌细胞株MCF-7为高表达RSK4基因的细胞,MDA-MB-231细胞为低表达RSK4基因的细胞,两者均可作为人乳腺癌细胞中研究RSK4基因的实验材料。
OBJECTIVE: To investigate the expression of ribosomal S6 kinase 4 (RSK4) tumor suppressor gene in human breast cancer cell lines MDA-MB-231, T47D, Bcap37 and MCF-7 and to screen for the expression of RSK4 gene in breast cancer cells . Methods: The expression of RSK4 in MDA-MB-231, T47D, Bcap-37 and MCF-7 breast cancer cell lines was detected by RT-PCR and Western blotting at mRNA and protein levels. Results: The expression of RSK4 gene was detected in human breast cancer cell lines MDA-MB-231, T47D, Bcap37 and MCF-7. Among them, the expression of RSK4 in MCF-7 cells was the highest 2.9-fold. The expression level of RSK4 protein in MCF-7 cells was the highest. Compared with the other three cell lines, the difference was statistically significant (P <0.001). The results of RT-PCR and Western blotting were consistent, with a high degree of correlation (r = 0.766, P <0.001). CONCLUSION: Human breast cancer cell line MCF-7 is a cell with high expression of RSK4 gene. MDA-MB-231 cell line is a cell with low expression of RSK4 gene, both of which can be used as experimental materials for RSK4 gene research in human breast cancer cells.