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目的 探讨食管癌高发区人群食管上皮癌变过程中的早期分子改变及早期癌变机理。方法 自食管癌高发区采集食管黏膜上皮细胞 ,碘化丙啶 (propidiumlodide ,PI)染色进行DNA含量及倍体测定 ;对各基因蛋白进行间接免疫荧光标记 ,采用流式细胞仪 (flowcytometry,FCM)进行定量检测。结果 DNA含量在癌形成时明显增高 ,二倍体细胞显著减少 ,而异倍体细胞明显增多 ,在癌细胞组异倍体率为 84.2 % ;同时p5 3蛋白有明显积聚 ,而抑癌基因p16有明显缺失。在癌细胞组p5 3及cyclinD1蛋白表达的阳性率均为 10 0 % (5 /5、6 /6 )。结论 在癌形成早期 ,DNA含量及异倍体率增加 ,癌基因cyclinD1表达增高 ,抑癌基因p16缺失及p5 3蛋白积聚。食管上皮癌形成时已有多个分子事件发生。
Objective To investigate the early molecular changes and early carcinogenesis of esophageal cancer esophageal cancer in esophageal carcinogenesis. Methods The esophageal mucosa epithelial cells were collected from the high incidence area of esophageal cancer. DNA content and ploidy were determined by propidium iodide (PI) staining. Indirect immunofluorescence labeling of each gene was carried out. Flow cytometry (FCM) Quantitative detection. Results DNA content was significantly higher in the formation of cancer, diploid cells decreased significantly, and aneuploid cells increased significantly in the aneuploidy of cancer cells was 84.2%; at the same time p5 3 protein accumulation, and tumor suppressor gene p16 There is a clear lack of. The positive rate of p5 3 and cyclinD1 protein in cancer cell group was 100% (5 / 5,6 / 6). Conclusion In the early stage of carcinogenesis, DNA content and aneuploidy rate are increased, the expression of oncogene cyclinD1 is increased, p16 deletion and p5 3 protein are accumulated. Multiple molecular events have occurred in the formation of esophageal epithelium.