论文部分内容阅读
目的 研究IL 1的Ⅰ型受体 (IL 1RⅠ )在大鼠颈动脉体内的表达和亚细胞定位。 方法 灌流固定的正常大鼠颈动脉体组织 ,用冰冻置换法包埋 ,超薄切片 ,胶体金免疫组织化学染色 ,电镜下观察。 结果 IL 1RⅠ样免疫反应阳性产物主要存在于主细胞 ,胶体金颗粒分布在胞膜、胞浆和胞浆中的细胞器及细胞核。支持细胞和毛细血管内皮细胞的胞浆中也可见少量免疫胶体金颗粒。 结论 IL 1RⅠ在大鼠颈动脉体特别是主细胞中有较强表达 ,此结果为进一步研究颈动脉体作为细胞因子化学感受器的可能性提供形态学基础。
Objective To study the expression and subcellular localization of IL - 1 receptor type 1 (IL 1 RI) in rat carotid artery. Methods The carotid arteries of normal rats were perfused and fixed. The frozen sections of the carotid body were embedded with cryopreserved sections. The sections were stained with gold pellet and immunohistochemically by colloidal gold staining. Results IL 1R Ⅰ-like immunopositive products mainly existed in the major cells and the colloidal gold particles distributed in the membrane, cytoplasm and cytoplasm of organelles and nuclei. A small amount of immunogold gold particles were also found in the cytoplasm of supporting cells and capillary endothelial cells. CONCLUSION: IL 1 R Ⅰ is strongly expressed in the carotid body of rat, especially in the main cell. The results provide a morphological basis for further study on the possibility of carotid body as a cytokine chemoreceptor.