论文部分内容阅读
目的研究西洋参总皂苷乙酸水解产物的化学成分。方法采用溶剂萃取、硅胶柱色谱及制备型HPLC色谱方法分离纯化西洋参总皂苷乙酸水解产物,通过化学和波谱技术鉴定化合物的结构。结果从西洋参总皂苷乙酸水解产物中分离得到11个化合物,分别为人参皂苷-Rk1(Ⅰ)、人参皂苷-Rs6(Ⅱ)、人参皂苷-Rs7(Ⅲ)、人参皂苷-Rk2(Ⅳ)、异人参皂苷-Rh3(Ⅴ)、3-O-β-D-glucopyranosyl(1→2)-glucopyransyl-3β,12β,20(S)-trihydroxy-25-hy-droperoxy-dammar-23(E)-ene(Ⅵ)、三七皂苷B1(Ⅶ)、20(S)-人参皂苷-Rh1(Ⅷ)、20(R)-人参皂苷-Rh1(Ⅸ)、拟人参皂苷-F11(Ⅹ)、6-O-β-D-(6-′acetyl)-glucopyranosyl-24-ene-dammar-3β,6α,12β,20S-tetraol(Ⅺ)。结论化合物Ⅰ~Ⅲ、Ⅴ~Ⅶ和Ⅹ为首次从西洋参水解产物中分得。
Objective To study the chemical constituents of acetic acid hydrolysates from Panax quinquefolium. Methods Solvent extraction, silica gel column chromatography and preparative HPLC were used to separate and purify the acetic acid saponin hydrolysate. The structures of the compounds were identified by chemical and spectral techniques. Results Eleven compounds were isolated from the ginsenoside saponin acetic acid hydrolysate. They were ginsenoside-Rk1(I), ginsenoside-Rs6(II), ginsenoside-Rs7(III), ginsenoside-Rk2(IV), and iso Ginsenoside-Rh3(V),3-O-β-D-glucopyranosyl(1→2)-glucopyransyl-3β,12β,20(S)-trihydroxy-25-hy-droperoxy-dammar-23(E)-ene (VI), Notoginsenoside B1 (VII), 20(S)-ginsenoside-Rh1(VIII), 20(R)-ginsenoside-Rh1(IX), Pseudomonas saponin-F11(X), 6-O -β-D-(6-’acetyl)-glucopyranosyl-24-ene-dammar-3β, 6α, 12β, 20S-tetraol (XI). Conclusions Compounds I-III, V-VII and X are firstly obtained from the hydrolysate of American ginseng.