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辽河油田冷 43块油藏所产原油含胶质沥青质 37%～ 42 % ,含蜡 5 % ,粘度 9.6～ 43Pa·s(5 0℃ ) ,密度 0 .96 0～ 0 .975 g/cm3 ,已超出了国外某些标准规定的微生物采油适用范围。用优选的适应温度范围为 40～ 6 0℃的两种菌假单胞菌 (Pseudomonassp .)LH 18和短杆菌 (Brevibacteriumsp .)LH 2 1的等量混合物、培养基 ,分别与该区块5口油井所产原油在 48℃摇床发酵 48小时 ,使原油粘度降低 2 6 %～ 6 5 % ,发酵液表面张力下降 10 %～ 19% ,pH值由中性变为弱酸性 ,表明在原油发酵过程中有表面活性物质和酸生成。在该区块蒸汽吞吐效果很差的同层位 5口井的近井地带注入营养液和混合菌液 ,共 7井次 ,单井一次注入菌液 0 .5～ 1.2t,注入后采用机械抽油。 5口井中有 3口井既增产油又减产水 ,1口井 (间开井 )连续生产。这 4口特重油井单井微生物吞吐试验获得成功。另 1口井产液量和产水量均大幅度上升 ,增产油量极微。同一层位的 5口井微生物采油效果相差很大 ,特别是微生物疏通出水层或通道 ,其原因有待研究
The crude oil produced in 43 cold reservoirs of Liaohe Oilfield contains 37% ~ 42% of asphaltene, 5% wax, viscosities of 9.6 ~ 43 Pa · s (50 ℃) and densities of 0 .96 0 ~0.975 g / cm3 , Has exceeded the scope of some foreign standards of microbial oil recovery. Using a preferred mixture of two Pseudomonas sp. LH 18 and Brevibacterium sp. LH 2 1 adapting to a temperature range of 40 to 60 ° C, the culture medium was incubated with this block 5 The crude oil produced by the oil well wells was shaken for 48 hours at 48 ° C to reduce the viscosity of the crude oil by 26% to 65% and the surface tension of the fermentation broth by 10% to 19%. The pH value changed from neutral to weak acidity, During the fermentation there are surface active substances and acid formation. A total of 7 wells were injected with nutrient solution and mixed bacterial liquid in the near-well zone of 5 wells in the same layer with poor steam huff and puff in the block. The single well was injected with 0.5 to 1.2 t of bacterial liquid once, Sucker. There are 3 wells in 5 wells that increase both oil production and water production. One well (open well) is continuously produced. The four special heavy oil well single well microbiological throughput test was successful. The other one well fluid production and water production have increased substantially, increasing oil production is minimal. The results of microbial oil recovery in the five wells in the same layer are quite different, especially for the reason that microorganisms dredge the water layer or channel