论文部分内容阅读
目的观察顺铂诱导不同性别小鼠所致急性肝肾损伤差异,并进一步探讨不同性别小鼠肝肾脏中谷胱甘肽(GSH)含量差异。方法选择ICR雌性和雄性小鼠,分别一次性腹腔注射0、10、20和40 mg/kg的顺铂,通过体重的变化确定小鼠的中毒情况,通过检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性来观察顺铂诱导的急性肝损伤差异,通过检测血清尿素氮(BUN)的含量来确定顺铂诱导的急性肾损伤差异,采用比色法检测雌雄小鼠肝肾中GSH的含量。结果顺铂能够引起不同性别的小鼠体重下降(P<0.01),血清ALT和AST水平明显上升(P<0.001,P<0.01),但是没有性别差异;顺铂诱导BUN明显升高(P<0.05,P<0.001),且具有性别差异(P<0.05,P<0.01,P<0.001)。顺铂(10和20 mg/kg)引起雄性肝脏中GSH含量明显升高(P<0.01,P<0.001),而在40 mg/k降低,与阴性对照组没有明显区别。顺铂(40 mg/kg)引起雌性肝脏GSH含量明显升高(P<0.001)。顺铂引起雌雄小鼠肾脏GSH含量明显升高(P<0.05,P<0.01,P<0.001),且不同性别的差异也有统计学意义(P<0.05)。结论顺铂能够诱导明显的急性肝肾损伤。其中诱导的肝损伤在雌雄小鼠体内并无差异,而顺铂引起的肾损伤在雄性小鼠体内比雌性小鼠更严重。在毒性剂量下,GSH含量在小鼠肝脏没有性别差异,而肾脏具有性别差异,其中在雌性小鼠中GSH含量明显高于雄性。
Objective To observe the difference of acute liver and kidney injury induced by cisplatin in different sex mice and to further explore the difference of glutathione (GSH) content in liver and kidney of different sex mice. Methods ICR female and male mice were selected and intraperitoneally injected with 0, 10, 20 and 40 mg / kg of cisplatin respectively. The mice were poisoned by changes in body weight. Serum levels of alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) activity to observe the cisplatin-induced acute liver injury differences, by detecting serum urea nitrogen (BUN) content to determine cisplatin-induced acute kidney injury differences, using colorimetric detection of male and female mice The content of GSH in liver and kidney. Results Cisplatin could cause weight loss in different sexes (P <0.01), serum ALT and AST levels increased significantly (P <0.001, P <0.01), but there was no gender difference; 0.05, P <0.001), and had gender differences (P <0.05, P <0.01, P <0.001). Cisplatin (10 and 20 mg / kg) caused a significant increase in GSH levels in male livers (P <0.01, P <0.001), but decreased at 40 mg / K, with no significant difference from the negative controls. Cisplatin (40 mg / kg) caused a significant increase in GSH levels in female livers (P <0.001). Cisplatin caused renal GSH levels in both male and female mice were significantly increased (P <0.05, P <0.01, P <0.001), and gender differences were also statistically significant (P <0.05). Conclusion Cisplatin can induce obvious acute liver and kidney injury. The induced liver injury did not differ between male and female mice, whereas the renal damage caused by cisplatin was more severe in male mice than in female mice. At toxic doses, GSH levels did not have sex differences in the liver of mice, while the kidneys had sex differences, with GSH levels significantly higher in males than in males.