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目的对一起食物中毒事件的病原菌进行调查检测,为食物中毒提供诊断依据。方法根据流行病学调查,采集相关标本,用食源性致病菌的实时荧光定量PCR试剂盒筛检,阳性者再用国标法进行致病菌培养和生化鉴定。结果综合流行病学资料和实验室检测结果,中毒是由于食用了被阪崎肠杆菌污染的变质饮料引起。结论荧光定量PCR检测特异性和敏感性高,检测时间短,通过筛查可以很快指向可疑食物,节省人力物力。建议国家在食物中毒诊断标准的修订中,将核酸检测方法与传统培养方法相结合,提高实验室诊断的效率。
Objective To investigate and test the pathogens of food poisoning incidents to provide the basis for diagnosis of food poisoning. Methods According to the epidemiological investigation, relevant specimens were collected and screened by real-time fluorescence quantitative PCR kit of food-borne pathogens. The positives were then tested for pathogen culture and biochemical identification by GB method. Results According to the epidemiological data and laboratory test results, the poisoning was caused by consumption of the deteriorated beverage contaminated with Enterobacter sakazakii. Conclusion Fluorescent quantitative PCR has the advantages of high specificity and sensitivity, short detection time, quick screening towards suspicious foods and saving manpower and material resources. It is suggested that in the revision of the diagnostic criteria for food poisoning, the state should combine the nucleic acid detection method with the traditional culture method to improve the efficiency of laboratory diagnosis.