【摘 要】
：
Plant rubisco consists of eight large subunits (55 kD) encoded by chloroplast gene and eight small subunits (15 kD) encoded by nuclear gene. There are abundant
【机 构】
：
State Key Laboratory of Biomembrane and Membrane Biotechnology
论文部分内容阅读
Plant rubisco consists of eight large subunits (55 kD) encoded by chloroplast gene and eight small subunits (15 kD) encoded by nuclear gene. There are abundant cysteine residues that do not form disulfide bonds in native rubisco. Differential scanning calorimetry has been used to study some plant rubisco and suggested an irreversible two-state denaturation due to the high cooperativity in subunits. By comparing the data from circular dichroism, fluorescence, differential scanning calorimetry, SDS electrophoresis, and activity assays in the absence or presence of DTT, we suggest that the formation of disulfide bonds in subunits during the early thermal unfolding may increase the thermal stability and the thermal unfolding cooperativity of rubisco.
其他文献
目的 了解两种不同毒力结核杆菌的纯化蛋白衍生物刺激下人单核-巨噬细胞(THP-1)凋亡的差异及其与Toll样受体-2(TLR-2)的相关性.方法 分别用等浓度的高毒力结核杆菌衍生蛋白(H
The Linnaean system provides ultimate means governing biological nomenclature and classification. With series of modification, this system has admirably served
Using immunohistochemistry, in situ hybridization, Western blot and TUNEL methods, we have studied the expression of Fas/FasL, Bcl-2/Bax and caspase-3in the cor
Since brain tumors endanger peoples living quality and even their lives, the accuracy of classification becomes more important. Conventional classifying techniques are used to deal with those datasets
A cDNA library with genomic complete coverage is a powerful tool for functional genomic studies. For studying the functions of rice genes on a large scale, a no
The DNA-binding activity of heat shock transcription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific to CaM
Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (Eps) inducing cell membrane pored (electroporation) are used as a means of deliv
The three-dimensional structure of Periplaneta fuliginosa densovirus (pfDNV) is determined at 2.3 nm resolution using the techniques of cryo-electron microscopy
Nuclear factor NF-κB is believed to play an important role in regulating the production of matrix met-alloproteinases (MMPs), which induce atherosclerosis, res
The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosom