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报道由肉苁蓉(Cistanchedeserticola)不同部位诱导出愈伤组织,并筛选出适合于愈伤组织快速生长的最佳培养条件。结果表明:幼芽愈伤组织诱导率较种子高;愈伤组织生长的基本培养基以B5最好;适宜的激素种类和配比为IAA0.5mg/L和KT2mg/L。适宜的培养条件为pH6.0。
Callus was induced from different parts of Cistanche mesenterica, and the optimal culture conditions suitable for rapid growth of callus were screened out. The results showed that the callus induction rate of young shoots was higher than that of seeds. The best medium for callus growth was B5. The appropriate hormones and ratios were IAA 0.5 mg/L and KT 2 mg/L. A suitable culture condition is pH 6.0.