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目的探讨孕妇胎盘中增殖细胞核抗原(PCNA)、P27的表达情况与妊娠期糖尿病发病的相关性。方法依据第8版《妇产科学》妊娠期糖尿病诊断标准,随机选择2016年1-6月吉林大学第二医院收治的妊娠期糖尿病患者60例为研究组,同期随机选取同年龄阶段的60例健康孕妇为对照组。入院后治疗前取空腹静脉血测空腹血糖(FBG),计算分娩前BMI等指标,胎盘娩出后15 min内取胎盘母体面近脐带根部直径3 cm范围内组织约1 cm~3大小用于免疫组化。检测所有研究对象FBG、BMI等水平,采用免疫组织化学技术显微镜下观察胎盘中PCNA、P27表达。结果研究组与对照组孕妇胎盘中PCNA、P27表达相比较差异有统计学意义(P<0.05),两组孕妇在年龄、分娩前BMI比较差异无统计学意义(P>0.05),两组孕妇入院空腹血糖差异有统计学意义(P<0.05)。结论 PCNA的升高、P27的降低与妊娠期糖尿病的发生存在相关性。
Objective To investigate the correlation between the expression of proliferating cell nuclear antigen (PCNA) and P27 in placenta of pregnant women and the incidence of gestational diabetes mellitus. Methods According to the diagnostic criteria of gestational diabetes in the 8th edition of Obstetrics and Gynecology, 60 cases of gestational diabetes mellitus admitted in the Second Hospital of Jilin University from January to June in 2016 were randomly selected as study group. 60 cases of the same age were randomly selected in the same period Healthy pregnant women as control group. Fasting blood glucose (FBG) was measured before admission. The BMI and other indexes before delivery were calculated. Within 15 minutes after delivery, the placenta was taken and the size of the tissue was approximately 1 cm ~ 3 within 3 cm of the root of the umbilical cord. Tissue. All subjects were tested for FBG, BMI and other levels. Immunohistochemistry was used to observe the expression of PCNA and P27 in the placenta under the microscope. Results There were significant differences in the expression of PCNA and P27 in the placenta between the study group and the control group (P <0.05). There was no significant difference in the age and BMI before delivery between the two groups (P> 0.05) There was a significant difference in fasting blood glucose admission (P <0.05). Conclusions There is a correlation between the increase of PCNA and the decrease of P27 in gestational diabetes mellitus.