This research focuses on analyzing attitudes of Romanticism and Enlightenment in Pride and Prejudice through studying the relationship between Elizabeth Bennet and Mr. Darcy and the marriage between Mr. Collins and Charlotte Lucas. Current research seems to have paid little attention on the contrast between attitudes Romanticism and Enlightenment in Pride and Prejudice. Therefore, this study is valuable in narrowing down the scope of research to the contrast between them. The research approach applied in this dissertation is documentary research method. This paper analyzes attitudes of Romanticism and Enlightenment from two aspects: one is the relationship between Elizabeth Bennet and Mr. Darcy; the other is the marriage between Mr. Collins and Charlotte Lucas. The findings from this research provide evidence that attitudes of valuing individualism and feeling over reason are indicated in Elizabeth Bennet and Mr. Darcy clearly; besides, the attitudes of valuing reason and self-control are revealed in Mr. Collins and Charlotte Lucas successfully. The main conclusion drawn from this study is that Elizabeth Bennet and Mr. Darcy think more highly of spiritual love in human relationship but Mr. Collins and Charlotte Lucas focus more on money and materials in marriage. This dissertation recommends that readers should develop their awareness of thinking about characters’ relationships critically in literature works; in addition, this study can be very useful if future researchers want to analyze Romanticism and Enlightenment in Jane Austen’s other works.