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目的:研究大鼠体内不同肠道菌群状态的改变对其非酒精性脂肪性肝病(NAFLD)发生发展的影响,为菌群失调动物模型的造模提供新方法,也为NAFLD在肠道菌群方面的防治提供可靠的理论依据。方法:50只雄性SD大鼠随机分为5组,其中3组使用高剂量抗生素头孢曲松溶液灌胃,建立肠道菌群失调模型后分为C组、D组和E组,C组饲以普通饲料、D组为高脂饲料、E组高脂饲料喂养同时使用益生菌灌胃,此3组在喂养期间,继续饮用低浓度头孢曲松溶液以保持肠道菌群失调状态;另2组生理盐水灌胃,作为肠道菌群正常模型,分为A组和B组,分别饲以普通饲料、高脂饲料。12周后收集标本,检测血脂、肝功能指标,行病理组织学检查明确肝组织病变程度,采用ELISA法测定血清脂联素水平。结果:同为普通饲料喂养的A组肝脏正常,而C组大鼠则出现少量的脂肪空泡。高脂饲料喂养的大鼠均造模NAFLD成功,B组肝脏为单纯性脂肪肝改变,抗生素作用的D组出现脂肪性肝炎(NASH),而益生菌干预的E组肝脏虽有炎症改变,但病变程度较D组大为改善。除D组血清脂联素水平明显降低外(P<0.05),其余各组大鼠均与A组无明显差异(P>0.05)。结论:肠道菌群的变化与NAFLD的发展存在明显的相关性,肠道菌群越紊乱,NAFLD病变程度越严重。微生态制剂可调节肠道菌群构成、改善NAFLD病变;血清脂联素浓度可能受血脂和肠道菌群双重影响,对肝脏起保护性作用,当NAFLD病变进展到NASH阶段时出现明显降低。
OBJECTIVE: To study the effects of different intestinal microflora changes in rats on the development of non-alcoholic fatty liver disease (NAFLD), and to provide a new method for modeling animal models of flora imbalance. Group prevention and control to provide a reliable theoretical basis. Methods: Fifty male Sprague-Dawley rats were randomly divided into five groups, of which three were given gavage with high-dose antibiotic ceftriaxone. The model of intestinal flora was divided into group C, group D and group E, group C Group D was fed with high-fat diet, group E was fed with high-fat diet and probiotic gavage. During the feeding, the three groups continued to drink low concentration of ceftriaxone solution to maintain the imbalance of intestinal flora. The other two groups Group normal saline gavage, as a normal model of intestinal flora, divided into group A and group B, were fed to normal feed, high-fat feed. After 12 weeks, the specimens were collected, blood lipid and liver function indexes were detected, and histopathological examination was performed to determine the degree of liver lesions. Serum adiponectin levels were measured by ELISA. Results: The liver of group A was normal fed with normal diet, while the group of fat of group C showed a small amount of fat vacuoles. Rats fed high-fat diets were successfully treated with NAFLD. The liver of group B was a simple fatty liver. Fatty hepatitis (NASH) was observed in group D of antibiotics. However, although the inflammation of group E was changed by probiotics, The degree of lesion was much better than that of group D. Except D group, serum adiponectin levels were significantly lower (P <0.05), the rest of the rats in each group and A group no significant difference (P> 0.05). Conclusion: There is a significant correlation between the change of intestinal flora and the development of NAFLD. The more disorganized intestinal flora and the more serious the pathological changes of NAFLD. The probiotics can regulate the composition of intestinal flora and improve the pathological changes of NAFLD. The concentration of serum adiponectin may be affected by both lipid and intestinal flora and play a protective role on the liver, which is obviously reduced when NAFLD progresses to NASH stage.