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目的探讨肾组织Ⅳ型胶原含量和过敏性紫癜性肾炎(HSPN)不同病理级别的关系。方法应用免疫组化染色法,检测了28例HSPN患儿肾组织Ⅳ型胶原的阳性表达和分布情况,并应用HPIAS高清晰度彩色分析系统对Ⅳ型胶原进行定量分析和研究。结果HSPN的Ⅳ型胶原在肾组织含量显著增加,以系膜区增加为主,并随着病理变化的加重,其阳性表达强度及面积百分比也相应增加。结论肾小球肾组织Ⅳ型胶原含量的增加是HSPN病理分级的重要参考指标之一。根据其含量多少,可间接反应病变进展及预后,具有较高的实用价值。
Objective To investigate the relationship between the content of collagen Ⅳ in renal tissues and the pathological grade of HSPN. Methods Immunohistochemical staining was used to detect the positive expression and distribution of type Ⅳ collagen in renal tissues of 28 HSPN patients. Quantitative analysis and study of type Ⅳ collagen using HPIAS high-resolution color analysis system was performed. Results The type Ⅳ collagen of HSPN increased significantly in the kidney and increased in the mesangial area. With the increase of pathological changes, the positive expression intensity and area percentage of type Ⅳ collagen increased accordingly. Conclusion The increase of collagen Ⅳ in glomerular nephropathy is one of the important reference indexes for the pathological grading of HSPN. According to its content how much, can indirectly reflect the progress and prognosis of disease, with high practical value.