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目的观察高度白酒和红葡萄酒对肝脏的不同作用;分别从能量代谢和氧化应激两个方面阐述高度白酒可引起小鼠的酒精性肝损伤;同时探讨槲芪散治疗酒精性肝损伤的药理学机制。方法将昆明种雄性小鼠随机分为4组,正常组、红葡萄酒组、白酒模型组和槲芪散治疗组。造模14 d后,取血,采用酶标仪比色法测定血清中丙二醛(malondialdehydehyde,MDA)的含量、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)的活性;取肝组织,进行HE染色,过碘酸-雪佛氏染色(schiffperiodicacidshiff,PAS染色);酶组织化学染色法检测葡萄糖-6-磷酸酶(glucose-6-phosphatase,G-6-P)、琥珀酸脱氢酶(succinate dehydrogenase,SDH)以及ATP酶(adenosinetriphosphatase,ATPase)的活性。结果白酒模型组MDA含量显著升高(P<0.05),抗氧化酶类SOD和CAT的活性显著减低(P<0.05);能量代谢的相关酶类ATPase、SDH以及G-6-P活性下降;红葡萄酒组HE染色显示红葡萄酒对肝脏无损伤。槲芪散能够显著降低因高度白酒造成的血清MDA含量(P<0.05),提高抗氧化酶SOD(P<0.05)和CAT(P<0.05)的活性;同时能量代谢相关酶的活性提高以及肝糖原储备增加。结论实验表明长期大量酒精摄入能够造成酒精性肝损伤,而红葡萄酒对肝脏无损伤;槲芪散对酒精性肝损伤有明显的干预作用。
Objective To observe the different effects of high liquor and red wine on the liver. To elucidate the effects of high liquor on alcoholic liver injury in mice from energy metabolism and oxidative stress, and to explore the pharmacology of Qiguqisan in treating alcoholic liver injury mechanism. Methods Kunming male mice were randomly divided into 4 groups: normal group, red wine group, liquor model group and QIQI treatment group. After 14 days of modeling, the blood was collected for determination of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) ). The liver tissues were harvested for hematoxylin-eosin (HE) staining and periodic acid-schiff staining (PAS staining). The levels of glucose-6-phosphatase (G-6- P), succinate dehydrogenase (SDH) and adenosine triphosphate (ATPase) activity. Results The content of MDA, the activities of antioxidative enzymes SOD and CAT in liquor model group were significantly increased (P <0.05), the activities of energy metabolism enzymes ATPase, SDH and G-6-P were decreased. HE staining of red wine group showed that red wine had no damage to the liver. QHD could significantly decrease the level of serum MDA (P <0.05), increase the activity of antioxidant enzyme SOD (P <0.05) and CAT (P <0.05); at the same time, the activity of energy metabolism- Glycogen reserves increased. Conclusion Experiments show that long-term intake of large quantities of alcohol can cause alcoholic liver injury, while red wine has no damage to the liver. Qu Qiqi San has a significant intervention on alcoholic liver injury.