双波长分光光度法是分光光度法领域中的一个新的重要的分支。1951年Chance首先设计成功双波长分光光度计,1968年已有商品仪器问世。1969年以来发展较快,1974年柴田正三等人撰写综述文章,介绍双波长分光光度法的原理与应用,近年来他们又撰写了一本专著,全书达200页,这是目前世界上第一本有关双波长法的著作。我国于七十年代后期才开始重视该法的介绍和评述,起步虽晚,但进展颇为迅速,已有一批研究成果,国内外《仪器分析》教科书中已有专门章节讲述此法。本文将以近几年的国内外文献为中心内容评述双 Dual-wavelength spectrophotometry is a new and important branch in the field of spectrophotometry. 1951 Chance first designed a successful dual-wavelength spectrophotometer, 1968 has been the introduction of commercial equipment. Since 1969, the development has been rapid. In 1974, Shibata Masatoshi et al. Wrote a review article introducing the principle and application of dual-wavelength spectrophotometry. In recent years, they wrote a monograph covering a total of 200 pages, which is the first in the world The book on dual wavelength law. China started to pay attention to the introduction and review of this law in the late 1970s. Although it started late, its progress was quite rapid. There have been a number of research achievements. There have been special chapters in instrumental analysis textbooks at home and abroad about this method. This article will review the domestic and foreign literature in recent years as the central content review