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采用荧光定量PCR技术对不同盐度胁迫下各时间点大菱鲆(Scophthalmus maximus)幼鱼肠、鳃中催乳素(PRL)基因和Na~+-K~+-ATPase α1两种基因的表达量进行检测。以盐度30为对照组,盐度5、10、40和50为实验组进行数据分析。结果显示,两种基因在两种组织中均有表达,且基因的表达量具有组织和时间特异性。肠组织PRL、Na~+-K~+-ATPase α1基因的表达量,在盐度50和5条件下,随胁迫时间的延长呈先升高后降低的变化趋势;鳃组织PRL基因表达量,在盐度50和盐度5条件下,随胁迫时间延长先升高后降低,而Na~+-K~+-ATPase α1基因表达量在低盐条件下(盐度5)没有显著变化,在高盐条件下(盐度50)随时间延长呈现先降低后升高的变化趋势。在肠组织中,两种基因存在极显著的协同作用,随着盐度的升高,两种基因的表达量都呈现先升高后降低的趋势,且相关系数均接近于1;在鳃组织中,在10–40盐度范围内,两种基因的表达存在明显的拮抗作用,当PRL基因的表达量呈现升高(或下降)趋势时,Na~+-K~+-ATPase α1基因的表达量呈现下降(或升高)趋势,且两种基因的相关系数均为负值。研究表明,PRL具有抑制Na~+/K~+-ATP酶活性的作用,为今后盐度胁迫分子调控机理研究提供理论依据。
The expression of PRL gene and Na ~ + -K ~ + -ATPase α1 gene in gut of Scophthalmus maximus at different salinity stress levels were detected by real-time PCR. Test. Salinity of 30 as a control group, salinity 5,10,40 and 50 for the experimental group data analysis. The results showed that both genes were expressed in both tissues, and the gene expression was tissue-specific and time-specific. The expression level of PRL and Na ~ + -K ~ + -ATPase α1 in intestinal tissue increased firstly and then decreased with the prolongation of stress time under the salinity of 50 and 5. The expression of PRL gene in gill tissue, Under the condition of salinity 50 and salinity 5, the expression of Na ~ + -K ~ + -ATPase α1 increased first and then decreased with prolonging of stress time, while the expression of Na ~ + -K ~ + -ATPase α1 had no significant change under low salt (salinity 5) Under the condition of high salt (salinity 50), the trend of decreasing first and then increasing with time prolonged. In the gut tissue, the two genes showed a very significant synergistic effect. With the increase of salinity, the expression of both genes increased first and then decreased, and the correlation coefficients were all close to 1. In gill tissues , The expression of both genes showed obvious antagonism in the range of 10-40 salinity. When the expression level of PRL gene showed an increasing (or decreasing) trend, the expression of Na ~ + -K ~ + -ATPase α1 The expression level showed a decreasing (or increasing) trend, and the correlation coefficients of the two genes were negative. The results showed that PRL could inhibit the activity of Na ~ + / K ~ + -ATPase and provide a theoretical basis for molecular regulation of salinity stress in the future.