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目的研究兰州地区重症肝炎患者TTV感染状况及基因型。方法采用TTV基因ORF1区套式PCR技术检测血清中TTVDNA,限制性内切酶进行酶切分型研究。结果57例重症肝炎患者血清中TTVDNA阳性率为36.8%(21/57),基因表现型的感染率依次为la型33.3%(7/21)、lb型23.8%(5/21)、2a型9.5%(2/21)、2b型9.5%(2/21)、1b/2a型14.3%(3/21)、1b/2b型9.5%(2/21)。结论兰州地区重症肝炎患者中有较高比率的TTV感染,重叠感染率高于单纯TTV感染率;血清中存在2种基因型、6种基因表现型,其中2b型、1b/2b型为首次发现,基因表现型呈现多样化。应对TTV的致病性进行深入研究。
Objective To study the status and genotype of TTV infection in patients with severe hepatitis in Lanzhou area. Methods TTV gene ORF1 nested PCR was used to detect TTVDNA in serum and restriction endonucleases. Results The positive rate of TTVDNA in serum of 57 patients with severe hepatitis was 36.8% (21/57). The prevalence of gene expression was 33.3% (7/21) of la, 23.8% (5/21) of lb, 9.5% (2/21) for type 2b, 9.5% (2/21) for type 2b, 14.3% (3/21) for type 1b / 2a and 9.5% (2/21) for type 1b / 2b. Conclusion There was a high rate of TTV infection in patients with severe hepatitis in Lanzhou area, and the overlap infection rate was higher than that of pure TTV infection. There were two genotypes and six gene phenotypes in serum, of which type 2b and 1b / 2b were the first found , Gene expression showed diversity. TTV pathogenicity should be carried out in-depth study.