论文部分内容阅读
采用cDNA PCR技术 ,从人胎盘cDNA文库DNA中克隆了人碱性成纤维细胞生长因子 (hbFGF)基因。用PCR突变法对其 5 端序列进行修饰 ,将天然和修饰后的hbFGF基因分别克隆至表达载体 pBV2 2 1,免疫印迹和SDS PAGE结果证明 ,经修饰后的基因在大肠杆菌DH5α中获得了表达 ,表达量占菌体总蛋白的 9%。
Human basic fibroblast growth factor (hbFGF) gene was cloned from human placenta cDNA library by cDNA PCR. The 5-terminal sequence was modified by PCR and the native and modified hbFGF genes were cloned into the expression vector pBV2 2 1, respectively. Western Blot and SDS PAGE results showed that the modified gene was expressed in E. coli DH5α , The expression of total bacterial protein accounted for 9%.