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本试验采用聚丙烯酰胺凝胶电泳法,检测了板栗和日本栗两个种的41个品种(系)叶片的过氧化物酶同工酶。结果表明,栗品种叶片的过氧化物同工酶可分为二大酶区,第Ⅰ酶区相对泳动率(Rf)范围为0.056～0.272,共检出6条酶带;第Ⅱ酶区Rf=0.478～0.647,共检出8条酶带。根据各酶带的出现频率,提出了粟品种叶片过氧化物酶同工酶的基本酶谱。并采用信息量凝聚聚类法,初步绘出了栗品种间的亲缘关系图,认为我国板栗与日本栗间的亲缘关系比较接近。
In this experiment, polyacrylamide gel electrophoresis was used to detect the peroxidase isoenzyme in 41 varieties (lines) of chestnut and Japanese chestnut. The results showed that peroxidase isozymes in chestnut cultivars were divided into two major enzyme zones, and the relative mobility (Rf) of zone Ⅰ was between 0.056 and 0.272. Six bands were detected. The second enzyme zone Rf = 0.478 ~ 0.647, a total of eight bands were detected. According to the appearance frequency of each enzyme band, the basic zymogram of peroxidase isozymes in the leaves of millet varieties was proposed. And the amount of information aggregation clustering method, initially drawn the genetic relationship between the chestnut varieties, that chestnut and Japanese chestnut between the genetic relationship is relatively close.