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目的:观察骨癌痛模型大鼠脊髓背根神经节中P2X3受体及其mRNA的表达变化,初步探讨其可能的意义。方法:60只雌性Wistar大鼠随机分为3组(每组n=20):空白对照(C)组;骨癌痛模型(CP)组,左胫骨内接种含2×105个Walker-256大鼠乳腺癌细胞的悬液10μl;假手术(S)组,左侧胫骨骨髓腔内注入等体积的生理盐水。在接种后第4、7、10、14、17、21天进行疼痛行为学测试,在第14、21天各组分别选10只大鼠,取背根神经节,5只行免疫组化染色,另5只抽提RNA行实时PCR,检测背根神经节中P2X3受体及其mRNA的表达。结果:CP组大鼠在胫骨内接种肿瘤后第10天开始出现痛觉过敏,第14～21天最为明显。CP组接种肿瘤后第14、21天患侧背根神经节神经元中P2X3受体免疫阳性细胞率明显增高(P<0.05),mRNA表达水平显著增高(P<0.05)。结论:骨癌痛模型大鼠存在痛觉敏化,可能与P2X3受体表达增高有关。
OBJECTIVE: To investigate the expression of P2X3 receptor and its mRNA in the dorsal root ganglion of rats with bone cancer pain model and to explore its possible significance. Methods: Sixty female Wistar rats were randomly divided into 3 groups (n = 20 each): blank control group (C); bone cancer pain model group (CP), left tibia inoculated with 2 × 105 Walker-256 large 10μl mouse breast cancer cell suspension; sham operation (S) group, the left tibial bone marrow cavity into an equal volume of saline. The pain behavior test was carried out on the 4th, 7th, 10th, 14th, 17th and 21st day after inoculation. On the 14th and 21st days, 10 rats in each group were selected and the dorsal root ganglions were taken. The immunohistochemical staining , And the other 5 RNA extraction real-time PCR detection of P2X3 receptor and mRNA expression in the dorsal root ganglion. Results: The rats in CP group developed hyperalgesia on the 10th day after inoculation of the tumor in the tibia, the most obvious on the 14th to 21st days. The number of P2X3 receptor positive cells in the dorsal root ganglion neurons increased significantly (P <0.05) and the mRNA expression level was significantly increased in the CP group (P <0.05) on the 14th and 21st day after tumor inoculation. Conclusion: There is pain sensitization in rat model of bone cancer pain, which may be related to the increase of P2X3 receptor expression.