论文部分内容阅读
目的 建立一个部分模拟临床病理的简便易得、经济有效的用于筛选和研制抗焦虑药的模型。方法 用间氯苯哌嗪 (mCPP)诱导产生焦虑 ,观察ddy小鼠和ICR小鼠在明暗箱的行为表现。结果 mCPP在SC 1- 4mg kg的剂量下即可显著降低ddy小鼠在明箱的活动次数 ,而对暗箱的活动次数影响不显著 ;1mg kg与 4mg kg组比较 ,在明箱的活动次数无显著差异 ;mCPP在SC 2 - 10mg kg的剂量下对ICR小鼠在明箱的的活动次数影响不太显著 ,只在10mg kg剂量下才显著 ;而对暗箱的活动次数却影响较大 ,在 2mg kg的剂量下即可显著降低活动次数。用安定对该模型进行验证 ,发现只用较小的样本量即可得出显著结果。结论 用ddy小鼠可以取代Wistar大鼠建立mCPP诱导焦虑的明暗箱模型 ,在样本量较小的情况下即可得到有效结果。所以是一个能够克服大鼠实验缺点的、简便易得且经济有效的用于筛选和研制抗焦虑药的良好模型。但用mCPP诱导的焦虑模型对小鼠的种属有选择性 ,ICR小鼠不适于上述模型。
Objective To establish a simple and easy-to-use, cost-effective model for screening and developing anxiolytic drugs that partially mimic clinical pathology. Methods The anxiety was induced by mCPP, and the behavior of ddy mice and ICR mice in the dark box was observed. Results In the dose of 1-40 mg SC, mCPP could significantly reduce the number of activities of ddy mice in the light box, but had no significant effect on the number of activities of the black box. Compared with the 4 mg kg group, Significant difference; mCPP in the dose of SC 2 - 10mg kg dose of ICR mice in the Ming box activity was not significant, only significant at 10mg kg dose; but the number of activities on the black box has a greater impact on the 2mg kg dose can significantly reduce the number of activities. The stability of the model was validated and found to yield significant results using only a small sample size. Conclusion Ddy mice can be used to replace the Wistar rats to establish mCPP-induced light and dark box model, and the effective results can be obtained when the sample size is small. Therefore, it is a good model for screening and developing anxiolytic drugs which is easy and cost-effective to overcome the shortcomings of the experiments in rats. However, the mCPP-induced anxiety model is selective for the species of the mouse, and ICR mice are not suitable for the above model.