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目的建立利咽含片中甘草酸的含量测定方法。方法采用反相高效液相色谱法(reversed phase high-performance liquid chromatography,RP-HPLC)测定利咽含片中甘草酸的含量。色谱柱为Welch ultimate XB-C18(200 mm×4.6mm,5μm);柱温:室温;流动相:甲醇-1%冰醋酸(63:37);流速:1 ml/min,检测波长:250 nm。结果甘草酸单铵进样量在0.438~8.760μg线性关系良好(r=0.999 5),平均加样回收率为98.7%,RSD为1.06%。本品每片含甘草以甘草酸计算,不得少于0.4 mg。结论采用RP-HPLC测定甘草酸含量简便、灵敏、准确,可用于该制剂的质量控制。
Objective To establish a method for the determination of glycyrrhizic acid in Liyan Lips. Methods The content of glycyrrhizic acid in Liyan Lips was determined by reversed phase high-performance liquid chromatography (RP-HPLC). The column was Welch ultimate XB-C18 (200 mm × 4.6 mm, 5 μm). The column temperature was at room temperature and the mobile phase was methanol-1% acetic acid (63:37). The flow rate was 1 ml / min and the detection wavelength was 250 nm . The results showed that the linearity of the sample was linear in the range of 0.438 ~ 8.760 μg (r = 0.999 5). The average recovery was 98.7% and the RSD was 1.06%. This product contains glycyrrhizin licorice per tablet calculation, not less than 0.4 mg. Conclusion Determination of glycyrrhizic acid by RP-HPLC content is simple, sensitive and accurate, and can be used for the quality control of the preparation.