论文部分内容阅读
原子力显微镜(AFM)的出现为免标记研究近生理环境下单个活体状态细胞的机械特性提供了新的技术手段.自20世纪90年代中期以来,研究人员在利用AFM测量细胞机械特性方面开展了大量研究,结果表明细胞机械特性是一个新的免标记生物标志物(可有效指示细胞生理状态的变化),加深了人们对癌症等重大疾病的认识,促进了细胞生物力学的发展.然而,现有的AFM单细胞机械特性研究主要集中在体外培养的细胞系,由于体内体外环境的巨大差异导致测量结果难以完全反映人体内的真实情况.特别是在精准医疗时代,需要对来自患者的原代细胞进行测试分析以实现疾病的个性化诊治.因此发展直接对临床患者原代细胞(癌变细胞和正常细胞)机械特性进行离体检测的方法具有潜在的转化医学实际意义.本文结合作者在基于AFM的淋巴瘤病例细胞机械特性测量与表征方面的研究工作,介绍了AFM测量细胞机械特性的原理与方法,总结了近年来AFM在检测原代细胞机械特性方面的进展,并对其面临的问题和挑战进行了讨论.
The emergence of atomic force microscopy (AFM) has provided a new technology for label-free study of the mechanical properties of cells in a single living state in a near physiological environment. Since the mid-1990s, researchers have been conducting extensive research on measuring cellular mechanical properties using AFM Studies have shown that the mechanical properties of cells is a new marker-free biomarker (which can effectively indicate changes in cell physiological status), deepen the understanding of people with major diseases such as cancer and promote the development of cell biomechanics.However, existing Of the AFM single-cell mechanical properties of the study focused on cell lines cultured in vitro and in vivo due to the huge differences between the measurement results can not fully reflect the true situation in the human body, especially in the era of precision medical needs from patients with primary cells To carry out the test analysis to realize the personalized diagnosis and treatment of the disease.Therefore, to develop a method that directly examines the mechanical characteristics of primary patient’s cells (cancer cells and normal cells) in vitro has the potential practical significance of translational medicine.In this paper, Research workers in the measurement and characterization of cellular mechanical properties of lymphoma cases The principle and method of measuring mechanical characteristics of cells by AFM were introduced. The progress of AFM in detecting the mechanical characteristics of primary cells in recent years was summarized. The problems and challenges faced by AFM were also discussed.