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目的 :研究ras基因、p53基因突变与喉鳞状细胞癌发病的关系。方法 :应用多聚酶链反应 单链构像多态性分析 (PCR SSCP)方法分析 4 0例喉鳞状细胞癌H ras ,K ras ,和N ras基因的第 12密码子 ,p53基因第 5,7外显子中点突变的情况。结果 :H ras基因突变 9例 ,突变率为 2 2 5% ( 9 4 0 ) ;K ras基因突变 8例 ,突变率为 2 0 % ( 8 4 0 ) ,N ras基因突变 5例 ,突变率为 12 5% ( 5 4 0 ) .p53基因第 5外显子突变 7例 ,突变率为17 5% ( 7 4 0 ) ;第 7外显子突变 9例 ,突变率为 2 2 5% ( 9 4 0 )。 5例发现有两种基因突变 ,且均表现为ras基因和p53外显子改变。结论 :ras基因 ,p53基因的改变在喉鳞状细胞癌的发生中共同起作用
Objective: To study the relationship between ras gene and p53 gene mutations and the pathogenesis of laryngeal squamous cell carcinoma. Methods: The 12th codon of H ras, K ras and N ras gene in 40 cases of laryngeal squamous cell carcinoma was analyzed by polymerase chain reaction single strand conformation polymorphism (PCR SSCP) Midpoint mutations in exon. RESULTS: There were 9 H ras mutations (225%), 8 K ras mutations (20%), 80 mutations (N 040) and 5 mutations The mutation rate of exon 5 was 125% (54O). The mutation rate of exon 5 was 17 5% (7 40). The mutation of exon 7 was found in 9 cases with the mutation rate of 225% 9 4 0). Five cases were found to have two gene mutations, and all showed ras gene and p53 exon changes. Conclusion: The alterations of ras and p53 genes work together in the pathogenesis of laryngeal squamous cell carcinoma