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目的 :研究食管癌旁上皮p5 3和增殖细胞核抗原 (PCNA)的表达及意义。方法 :用免疫组化法检测 32例食管正常黏膜上皮、12 4例增生上皮及 12 0例不典型增生上皮中p5 3和PCNA的表达。结果 :正常食管黏膜上皮中无p5 3的积聚。增生上皮及不典型增生上皮中p5 3积聚率分别是 5 5 %和 79% ,二者之间差异有显著性 (P <0 .0 1)。PCNA的 5 0 %的阳性率分别是0 .8%和 31.7% ,差异亦有显著性 (P <0 .0 1)。结论 :p5 3的改变在上皮癌变前即已存在。p5 3积聚及PCNA可能成为判断食管上皮为癌前病变的客观标准之一
Objective: To study the expression and significance of p53 and PCNA in paracancerous epithelium of esophageal squamous cell carcinoma. Methods: The expressions of p5 3 and PCNA in 32 esophageal mucosal epithelia, 12 4 hyperplastic epithelium and 120 atypical hyperplastic epithelium were detected by immunohistochemistry. Results: There was no accumulation of p5 3 in normal esophageal epithelium. The accumulation rate of p5 3 in proliferative epithelium and atypical hyperplastic epithelium was 55% and 79%, respectively, with a significant difference between the two (P <0.01). The positive rates of 50% of PCNA were 0.8% and 31.7%, respectively, with significant difference (P <0.01). Conclusion: The change of p5 3 existed before epithelial canceration. p5 3 Accumulation and PCNA may be one of the objective criteria for determining esophageal epithelium as precancerous lesions