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目的从分子病理学水平探讨 HCV 基因型与 HCC 发生的关系。方法采用逆转录原位 PCR 法对95例 HCC 及其非癌组织中1b 型和2a 型 HCV 进行检测,同时采用 Okamoto 法对 HCC 组织 RNA 抽提物中 HCV 基因型加以验证。结果 HCC 中 HCV 阳性率为44%(42/95),检出3种 HCV 基因型,包括1b(13例,31%)、2a(23例,55%)、混合型(1b+2a,2例,5%)和3a(4例,10%),1b 型检出率(14%)与2a 型者(24%)无显著性差异(X~2=3.427,P>0.05),两型与 HCC 主要病理学参数的相关性相似,2a 型阳性细胞在癌和非癌区的分布相似,而1b 型在癌组织多呈局灶分布,在非癌区多呈局灶/弥漫分布,HCC 中1b 型 HCV 的核-胞质型/核型阳性信号较2a 型者明显多见(X~2=4.073,P<0.05),去除逆转录并用RNase A 处理后,1b 型和2a 型 HCV 仍分别有5例和9例可见阳性信号。结论我国两种常见的 HCV 基因型与 HCC 发生的相关性相似,HCC 中 HCV 相关 DNA 及其与宿主基因组整合的可能性不能除外。
Objective To explore the relationship between HCV genotypes and the occurrence of HCC from the molecular pathological level. Methods Reverse transcription in situ PCR was used to detect type 1b and type 2a HCV in 95 cases of HCC and non-cancerous tissues. The Okamoto method was used to verify the HCV genotype in HCC tissues. Results The positive rate of HCV in HCC was 44% (42/95). Three HCV genotypes were detected, including 1b (13 cases, 31%), 2a (23 cases, 55%), mixed (1b+2a, 2) Cases, 5%) and 3a (4 cases, 10%), type 1b detection rate (14%) and type 2a (24%) were not significantly different (X~2=3.427, P>0.05). The correlations with the main pathological parameters of HCC were similar, and the distribution of type 2a positive cells was similar between cancerous and non-cancerous areas, whereas type 1b was mostly localized in cancer tissues, and mostly focal/diffusely distributed in non-cancerous areas. HCC The positive nuclear-cytoplasmic/karyotypic signals of type 1b HCV were significantly more common than those of type 2a (X~2=4.073, P<0.05). After removing reverse transcription and treating with RNase A, type 1b and type 2a HCV remained A positive signal was seen in 5 and 9 cases, respectively. Conclusion The correlation between the two common HCV genotypes in China and HCC is similar. The possibility of HCV-associated DNA and its integration with the host genome in HCC cannot be excluded.