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目的观察复方番石榴制剂对Hep G2细胞胰岛素抵抗(IR)的改善作用,及对α-淀粉酶和α-葡萄糖苷酶抑制作用。方法用高浓度胰岛素诱导Hep G2细胞建立胰岛素抵抗细胞模型,用葡萄糖氧化酶法检测复方番石榴制剂对IR-Hep G2细胞葡萄糖的消耗量;分别以可溶性淀粉和4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)为底物测定复方番石榴制剂对α-淀粉酶和α-葡萄糖苷酶活性的抑制率。结果与模型组比较,复方番石榴制剂(62.5,250,500μg·m L~(-1))显著增加了IR-Hep G2细胞对葡萄糖的消耗量(P<0.05,P<0.01)。在实验剂量范围内,复方番石榴制剂对α-淀粉酶和α-葡萄糖苷酶抑制作用均随浓度升高而抑制作用增加,IC50值分别为0.79 mg·m L~(-1)和0.48 mg·m L~(-1)。结论复方番石榴制剂可促进IR-Hep G2细胞对葡萄糖的消耗,改善Hep G2细胞对胰岛素的抵抗状态,并能体外抑制α-淀粉酶和α-葡萄糖苷酶活性。
Objective To observe the effect of compound guava preparations on insulin resistance (IR) in Hep G2 cells and its inhibitory effect on α-amylase and α-glucosidase. Methods Hep G2 cells were induced by high concentration of insulin to establish insulin resistance cell model. Glucose oxidase method was used to detect the glucose consumption of compound guava preparations on IR-Hep G2 cells. Soluble starch and 4-nitrophenol-α-D - glucopyranoside (PNPG) as substrate for the determination of compound guava preparations of α-amylase and α-glucosidase inhibition rate. Results Compared with the model group, the compound guava preparations (62.5, 250 and 500 μg · ml -1) significantly increased the glucose consumption of IR-Hep G2 cells (P <0.05, P <0.01). The inhibitory effect of compound guava preparations on the α-amylase and α-glucosidase increased with the increase of concentration in the experimental dose range, and the IC50 values were 0.79 mg · m L -1 and 0.48 mg · M L -1 (-1). Conclusion Compound guava preparations can promote the depletion of glucose in IR-Hep G2 cells, improve the insulin resistant status of Hep G2 cells and inhibit the activity of α-amylase and α-glucosidase in vitro.