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目的:研究磷酸化雷帕霉素靶蛋白(phosphorylated mammalian target of rapamycin,p-mTOR)、缺氧诱导因子(hypoxia-inducible factor,HIF)-1α在结直肠癌组织中的表达,探讨其与结直肠癌临床病理特征及预后的关系。方法:收集2007年6月至2013年12月在济南军区总医院普外科行手术切除并经病理证实的结直肠癌组织及癌旁正常黏膜组织标本,免疫组织化学方法检测p-mTOR、HIF-1α在63例结直肠癌组织、27例转移淋巴结组织、12例转移癌组织中的表达,以癌旁正常黏膜组织作对照,χ~2检验分析p-mTOR、HIF-1α的表达与临床病理特征的关系。p-mTOR和HIF-1α表达的相关性采用Spearman相关分析,Kaplan-Meier检验进行p-mTOR、HIF-1α的生存期分析。结果:在结直肠癌组织中,p-mTOR(50.80%vs 6.35%,χ~2=30.489,P<0.01)、HIF-1α(65.08%vs 9.52%,χ~2=47.323,P<0.01)的阳性率均显著高于正常结直肠组织;p-mTOR、HIF-1α表达与肿瘤的分期及远处转移及淋巴结转移相关,而且p-mTOR表达与HIF-1α表达呈正相关(r=0.345,P<0.01);p-mTOR阳性患者的无疾病进展生存期及总生存期均显著短于p-mTOR阴性患者(χ~2分别为4.584、4.557,均P<0.05)。结论:p-mTOR及HIF-1α在结直肠癌组织及转移癌组织中表达升高,其表达可以作为结直肠癌临床病理分期及预后的重要指标。
Objective: To investigate the expression of phosphorylated mammalian target of rapamycin (p-mTOR) and hypoxia-inducible factor (HIF) -1α in colorectal cancer Relationship between clinicopathological characteristics and prognosis in rectal cancer. Methods: Colorectal cancer tissues and adjacent normal mucosa specimens surgically removed from the Department of General Surgery, Jinan Military General Hospital from June 2007 to December 2013 were collected. The expressions of p-mTOR, HIF- 1α in 63 colorectal cancer tissues, 27 metastatic lymph node tissues and 12 metastatic cancer tissues. The normal mucosa adjacent tissues were used as control. The expressions of p-mTOR and HIF-1α were detected by χ ~ 2 test and their clinicopathological features Characteristics of the relationship. Correlation of p-mTOR and HIF-1α expression Spearman correlation analysis and Kaplan-Meier test were used to analyze the survival of p-mTOR and HIF-1α. Results: The expression of HIF-1α (65.08% vs 9.52%, χ ~ 2 = 47.323, P <0.01) in colorectal cancer tissues was significantly higher than that in the control group The positive rates of p-mTOR and HIF-1α were correlated with tumor stage, distant metastasis and lymph node metastasis, and the expression of p-mTOR was positively correlated with the expression of HIF-1α (r = 0.345, P <0.01). The disease-free survival and overall survival of patients with p-mTOR positive were significantly shorter than those with p-mTOR negative (χ ~ 2 = 4.584 and 4.557, all P <0.05). CONCLUSION: The expressions of p-mTOR and HIF-1α in colorectal cancer tissues and metastatic cancer tissues may be increased. The expression of p-mTOR and HIF-1α may be used as an important index for the clinical stage and prognosis of colorectal cancer.