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从32种不同产地、不同种类的腐植酸原料中分离微生物,以验证腐植酸原料中是否存在微生物;如果存在,则对其进行菌种鉴定。目前,关于腐植酸中存在微生物的研究鲜有报道。但是腐植酸中可能会存在一些有益的微生物,对其进行研究,可以为以后研究腐植酸与微生物两者之间的相互作用及应用奠定基础。试验结果表明,腐植酸原料中(除上海生化腐植酸外)存在细菌、放线菌、真菌,并且不同腐植酸中微生物的种类、数量不同,但优势菌大致相同;从中分离得到细菌优势菌8株、放线菌优势菌4株、真菌优势菌5株,通过菌落形态、菌体形态以及生理生化特征等方面鉴定各菌种。鉴定结果明确,它们属于短小杆菌属(Curtobacte-rium Yamada etc.,1972)、芽孢杆菌属的短小芽孢杆菌(B.pumilus)、灰褐类群阿拉伯链霉菌(S.arabicus Shibata etc.,1957)等。
Microorganisms were isolated from 32 different origins and different types of humic acid feedstocks to verify the presence of microorganisms in the humic acid feedstocks and, if present, identified. At present, little has been reported about the presence of microorganisms in humic acid. However, there may be some beneficial microorganisms in humic acid, which can be used as a basis for the future study on the interaction and application between humic acid and microorganisms. The results showed that there were bacteria, actinomycetes and fungi in the humic acid raw materials (except for the biochemical humic acid in Shanghai), and different types and quantities of microorganisms in different humic acids, but the dominant bacteria were almost the same. 4 strains of dominant actinomycetes and 5 strains of dominant fungi. All strains were identified by their colony morphology, morphology and physiological and biochemical characteristics. The identification results are clear, and they belong to the genus Curtobacterium-rium Yamada etc. (1972), B. pumilus from Bacillus, T. arabicus Shibata etc. (1957), etc. .