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我们在实验中已发现丝裂霉素 C(MMC)对培养的乳鼠心肌细胞在形态和功能方面都有明显的影响,可造成严重的细胞损伤,为了进一步探讨其作用机制,我们用~3H-TdR 掺入,液体闪烁计数法测定MMC 对心肌细胞 DNA 合成的影响,其实验结果报告如下。35只大白鼠乳鼠,取心、剪碎,胰蛋白酶消化成单细胞、采用贴壁分离法,除去心肌间质细胞,调节细胞浓度1×10~6/ml,将高纯度(约95%)的心肌细
We have found that mitomycin C (MMC) has a significant effect on the morphology and function of cultured neonatal rat cardiomyocytes and can cause severe cell damage. To further explore its mechanism of action, we use ~3H. -TdR incorporation, liquid scintillation counting method was used to determine the effect of MMC on myocardial DNA synthesis. The experimental results are reported below. Thirty-five male mice were harvested by heartbeat, cut into pieces, digested with trypsin into single cells, and adherently separated to remove myocardial interstitial cells. The cell concentration was adjusted to 1×10 6 /ml and the purity was high (about 95%). ) The myocardial fineness