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在0.0035～0.0045mol/L硫酸介质中,牛血清白蛋白(BSA)、人血清白蛋白(HSA)、卵白蛋白(OVA)和血红蛋白(HGB)等蛋白质以带正电荷的阳离子存在.它们能借助于静电引力和疏水作用力与配阴离子[HgI4]2--反应形成结合产物,此时将引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)显著增强,并且出现新的散射光谱.其最大RRS,SOS和FDS波长分别位于390,760和390nm附近.在一定范围内,三种散射增强(△IRRS,△ISOS和△IFDS)与蛋白质浓度成正比,方法具有高灵敏度,三种方法对于不同蛋白质的检出限分别在5.7～15.9ng/mL(RRS),8.2～15.4ng/mL(SOS)和11.2～22.1ng/mL(FDS)之间,均可用于痕量蛋白质的测定.本文研究了[HgI4]2-与蛋白质相互作用对RRS,SOS和FDS光谱特征和强度的影响,考察了适宜的反应条件,并以RRS为例考察了共存物质的影响,表明方法有良好的选择性.据此,利用[HgI4]2-与蛋白质的相互作用发展了一种用共振光散射技术、灵敏度高、简便、快速测定蛋白质的新方法.本方法可用于血清和人尿中总蛋白质的测定.
Proteins such as bovine serum albumin (BSA), human serum albumin (HSA), ovalbumin (OVA) and hemoglobin (HGB) are present as positively charged cations in 0.0035 to 0.0045 mol / L sulfuric acid medium Upon electrostatic attraction and hydrophobic interaction with the anion [HgI4] 2 -, the formation of a binding product will result in a significant enhancement of Resonance Rayleigh Scattering (RRS), Secondary Scattering (SOS) and Frequency Doubled Scattering (FDS) The new RRS, SOS and FDS wavelengths are located near 390, 760 and 390nm, respectively.The three kinds of scattering enhancement (△ IRRS, △ ISOS and △ IFDS) are proportional to the protein concentration within a certain range.The method has high sensitivity , The detection limits of different proteins were 5.7 ~ 15.9ng / mL (RRS), 8.2 ~ 15.4ng / mL (SOS) and 11.2 ~ 22.1ng / mL Protein.We studied the influence of the interaction between [HgI4] 2- and protein on the spectral characteristics and intensity of RRS, SOS and FDS, investigated the suitable reaction conditions and investigated the influence of coexisting substances by RRS as an example to show that the method There is a good selectivity.Accordingly, the use of [HgI4] 2-protein interaction has developed a Using resonance light scattering technology, a new method with high sensitivity, simple and rapid determination of protein can be used in the determination of total protein in serum and human urine.