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目的分析热休克蛋白70抗体(HSP70-Ab)与多环芳烃(PAH)相关肺癌的关系,为PAH相关肺癌发病机制及诊断指标的研究提供参考依据。方法采用Western Blot免疫印迹-酶联免疫吸附测定(ELISA)法分别检测PAH相关肺癌患者(肺癌组,52例)、非肿瘤性肺病者(配比组,34例)和健康者(健康组,30例)血浆中HSP70-Ab的抗体滴度。结果肺癌组不同抗体滴度组阳性检出率显著高于配比组和健康组(均P<0.05),配比组、健康组各滴度组间比较差异无显著性(均P>0.05)。logistic回归分析表明,接触苯并(a)芘[B(a)P]、同时接触粉尘及B(a)P与HSP70-Ab(1∶40)密切相关(分别为OR=5.1074,P=0.0433和OR=2.7874,P=0.0209)。结论HSP70可能与PAH相关肺癌患病有关,血浆HSP70-Ab高滴度(1∶40)可能是PAH相关肺癌的血清学标志物。
Objective To analyze the relationship between heat shock protein 70 antibody (HSP70-Ab) and polycyclic aromatic hydrocarbons (PAH) -related lung cancer and provide reference for the study of pathogenesis and diagnostic criteria of PAH-related lung cancer. Methods Fifty-two PAH-related lung cancer patients (lung cancer group, 52 patients), non-pulmonary lung disease patients (34 patients in the control group) and healthy individuals (healthy group) were detected by Western Blot and ELISA. 30 cases) antibody titers of HSP70-Ab in plasma. Results The positive rate of different antibody titers in lung cancer group was significantly higher than that in the matched group and the healthy group (all P <0.05). There was no significant difference between the titer group and the healthy group (all P> 0.05) . Logistic regression analysis showed that there was a close correlation between exposure to dust and B (a) P and HSP70-Ab (1:40) (OR = 5.1074, P = 0.0433 And OR = 2.7874, P = 0.0209). Conclusion HSP70 may be related to PAH-related lung cancer. High plasma HSP70-Ab titer (1:40) may be a serological marker of PAH-related lung cancer.