论文部分内容阅读
目的探讨粪便SEPT9(septin 9)基因甲基化检测在结直肠癌早期诊断中的应用价值。方法常规提取87例结直肠癌手术切除癌组织、癌旁正常结肠组织及其术前粪便DNA,应用巢式甲基化特异性PCR(nested methyl-ation specific PCR,nMSP)结合变性高效液相色谱(denatured high performance liquid chromatography,DHPLC)技术检测结直肠癌组织、癌旁组织及粪便SEPT9基因甲基化水平;比较结直肠癌组织和粪便中SEPT9甲基化检测在结直肠癌诊断中的敏感性和特异性。结果在87例结直肠癌中,癌组织SEPT9检出率为80.5%(70/87),癌旁组织检出率为8.0%(7/87),二者比较差异有统计学意义(P<0.01),特异性为91.9%;结直肠癌粪便SEPT9基因甲基化检测结果与组织检测结果基本一致。SEPT9基因甲基化与大于65岁老年患者和右半结肠癌显著相关。结论结直肠癌组织SEPT9基因甲基化异常发生率高,尤其是老年和右半结肠癌患者;粪便SEPT9基因甲基化异常可作为结直肠癌早期诊断的肿瘤标志物。
Objective To investigate the value of methylation detection of septin 9 gene in early diagnosis of colorectal cancer. Methods 87 cases of colorectal cancer tissues, adjacent normal colorectal tissues and their preoperative stool DNA were routinely extracted. Using nested methyl-specific PCR (nMSP) combined with denaturing high performance liquid chromatography (DHPLC) was used to detect the methylation level of SEPT9 gene in colorectal cancer tissues, paracancerous tissues and feces. The sensitivity of SEPT9 methylation detection in colorectal cancer tissues and faeces was compared in the diagnosis of colorectal cancer And specificity. Results In 87 cases of colorectal cancer, the detection rate of SEPT9 was 80.5% (70/87) in cancer tissues and 8.0% (7/87) in adjacent tissues, the difference was statistically significant (P < 0.01) and the specificity was 91.9%. The result of methylation of SEPT9 gene in colorectal cancer was consistent with the result of tissue test. SEPT9 gene methylation was significantly associated with older-than-65-year-old patients and right-sided colon cancer. Conclusion There is a high incidence of abnormal methylation of SEPT9 gene in colorectal cancer tissues, especially in elderly and right-sided colon cancer patients. Abnormal methylation of SEPT9 gene may be used as a tumor marker for early diagnosis of colorectal cancer.