方法:系取15—20克体重的雄性同种小鼠,固定于园筒固定籠中,露出并固定尾部,在皮下装入二电极,以每秒一次持续20毫秒的方形波电流刺激,先测定正常痛觉阈,电压从2—64伏特按倍数递增,直到动物当第一次或第二次发出反应叫声时作为痛觉反应指标,用药后仍按原电压强度连续四次刺激动物均不产生反应叫声者就作为阳性镇痛反应。 Methods: Male mice of 15-20 g body weight were fasted in a cylindrical cage. The tail was exposed and fixed. The two electrodes were subcutaneously infused at a square wave current of 20 milliseconds per second to stimulate Determination of normal pain threshold, the voltage from 2-64 volts in multiples of the increase until the animal when the first or second response to the call as a pain response index, after the medication is still the original voltage intensity of four consecutive animals did not stimulate Responder barks as a positive analgesic response.